dStorm / SIM / LSM / Lattice Lightsheet / RCM

Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

Reference: van de Linde et al., 2011

Olympus setup (2D, dual-color)

Microscope-body: Olympus IX-71 equipped with nosepiece stage (IX2-NPS; Olympus), inverted
Objective: Olympus 60x oil, NA 1.45 PlanApo, TIRF
Laser: 405 nm, 488 nm, 532 nm, 568 nm: 100 mW
642 nm: 200 mW
Cameras: 2 EMCCD, Andor iXon DU-897

High Power setup (2D, dual-color)

Microscope-body: Olympus IX-71 equipped with nosepiece stage (IX2-NPS; Olympus), inverted
Objective: Olympus APON 60XO TIRF oil, NA 1.49
Laser: 405 nm, 488 nm: 200 mW
514 nm, 558 nm: 500 mW
642 nm: 1000 mW
Cameras: 2 EMCCD Andor iXon Ultra DU-897

3D setup (2D, 3D, dual-color)

Microscope-body: Zeiss Axio Observer.Z1
Objective: Zeiss 63× LD C-Apochromat NA 1.15 water-immersion, 600 µm working distance
Zeiss Plan-Apochromat 100x NA 1,46 oil
Laser: 405 nm, 488 nm: 200 mW
532 nm: 500 mW
640 nm: 250 mW
Cameras: 2 EMCCD Andor iXon Ultra DU-897