Functional Proteomics (Warscheid Lab)

News from the lab

Bettina Warscheid elected as member of the DFG Grants Committee on RTGs

We are happy to announce that our Chair of Biochemistry II, Bettina Warscheid, has been elected by the DFG’s Senate as a new Member of the DFG Grants Committee and Senate Committee on Research Training Groups (RTGs).

Paper out: Regulation of Filamin C by Protein Phosphatase-1

A new study performed by Johannes Zimmermann and former lab mate Anja Schwäble from the lab of Bettina Warscheid together with Thomas Kokot from the lab of Maja Köhn investigates the role of protein phosphatase-1 (PP1) in skeletal muscle cells during mechanical stress.

Start of installation of the new mass spectrometers

The installation of the new mass spectrometers in the laboratory began today with the Orbitrap Ascend. Over the next two weeks, the Orbitrap Ascend and Orbitrap Astral will be brought to high vacuum, tested, calibrated and connected to the liquid chromatography systems.

Dewar, C. E., Oeljeklaus, S., Mani, J., Mühlhäuser, W. W. D., von Känel, C., Zimmermann, J., Ochsenreiter, T., Warscheid, B., & Schneider, A. (2022). Mistargeting of aggregation prone mitochondrial proteins activates a nucleus-mediated posttranscriptional quality control pathway in trypanosomes. Nature Communications, 13(1), 3084. https://doi.org/10.1038/s41467-022-30748-z
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Dewar, C. E., Oeljeklaus, S., Wenger, C., Warscheid, B., & Schneider, A. (2022). Characterization of a Highly Diverged Mitochondrial ATP Synthase F(o) Subunit in Trypanosoma Brucei. The Journal of Biological Chemistry, 298(4), 101829. https://doi.org/10.1016/j.jbc.2022.101829
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Morgenstern, M., Peikert, C. D., Lübbert, P., Suppanz, I., Klemm, C., Alka, O., Steiert, C., Naumenko, N., Schendzielorz, A., Melchionda, L., Mühlhäuser, W. W. D., Knapp, B., Busch, J. D., Stiller, S. B., Dannenmaier, S., Lindau, C., Licheva, M., Eickhorst, C., Galbusera, R., … Warscheid, B. (2021). Quantitative High-Confidence Human Mitochondrial Proteome and Its Dynamics in Cellular Context. Cell Metabolism, 33(12), 2464-2483.e18. https://doi.org/10.1016/j.cmet.2021.11.001
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Lill, P., Hansen, T., Wendscheck, D., Klink, B. U., Jeziorek, T., Vismpas, D., Miehling, J., Bender, J., Schummer, A., Drepper, F., Girzalsky, W., Warscheid, B., Erdmann, R., & Gatsogiannis, C. (2020). Towards the Molecular Architecture of the Peroxisomal Receptor Docking Complex. Proceedings of the National Academy of Sciences of the United States of America, 117(52), 33216-33224. https://doi.org/10.1073/pnas.2009502117
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Reimann, L., Schwäble, A. N., Fricke, A. L., Mühlhäuser, W. W. D., Leber, Y., Lohanadan, K., Puchinger, M. G., Schäuble, S., Faessler, E., Wiese, H., Reichenbach, C., Knapp, B., Peikert, C. D., Drepper, F., Hahn, U., Kreutz, C., van der Ven, P. F. M., Radziwill, G., Djinovi’c}}-Carugo, K., … Warscheid, B. (2020). Phosphoproteomics Identifies Dual-Site Phosphorylation in an Extended Basophilic Motif Regulating FILIP1-mediated Degradation of Filamin-C. Communications Biology, 3(1), 253. https://doi.org/10.1038/s42003-020-0982-5
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Maartensson, C. U., Priesnitz, C., Song, J., Ellenrieder, L., Doan, K. N., Boos, F., Floerchinger, A., Zufall, N., Oeljeklaus, S., Warscheid, B., & Becker, T. (2019). Mitochondrial Protein Translocation-Associated Degradation. Nature, 569(7758), 679-683. https://doi.org/10.1038/s41586-019-1227-y
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Topf, U., Suppanz, I., Samluk, L., Wrobel, L., Böser, A., Sakowska, P., Knapp, B., Pietrzyk, M. K., Chacinska, A., & Warscheid, B. (2018). Quantitative Proteomics Identifies Redox Switches for Global Translation Modulation by Mitochondrially Produced Reactive Oxygen Species. Nature Communications, 9(1), 324. https://doi.org/10.1038/s41467-017-02694-8
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Morgenstern, M., Stiller, S. B., Lübbert, P., Peikert, C. D., Dannenmaier, S., Drepper, F., Weill, U., Höß, P., Feuerstein, R., Gebert, M., Bohnert, M., van der Laan, M., Schuldiner, M., Schütze, C., Oeljeklaus, S., Pfanner, N., Wiedemann, N., & Warscheid, B. (2017). Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale. Cell Reports, 19(13), 2836-2852. https://doi.org/10.1016/j.celrep.2017.06.014
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Peikert, C. D., Mani, J., Morgenstern, M., Käser, S., Knapp, B., Wenger, C., Harsman, A., Oeljeklaus, S., Schneider, A., & Warscheid, B. (2017). Charting Organellar Importomes by Quantitative Mass Spectrometry. Nature Communications, 8, 15272. https://doi.org/10.1038/ncomms15272
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$Myofibrillar Z-discs Are a Protein Phosphorylation Hot Spot with Protein Kinase C (PKC$$\alpha$$) Modulating Protein Dynamics.$

Reimann, L., Wiese, H., Leber, Y., Schwäble, A. N., Fricke, A. L., Rohland, A., Knapp, B., Peikert, C. D., Drepper, F., van der Ven, P. F. M., Radziwill, G., Fürst, D. O., & Warscheid, B. (2017). Myofibrillar Z-discs Are a Protein Phosphorylation Hot Spot with Protein Kinase C (PKC$$\alpha$$) Modulating Protein Dynamics. Molecular & Cellular Proteomics : MCP, 16(3), 346-367. https://doi.org/10.1074/mcp.M116.065425
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Wrobel, L., Topf, U., Bragoszewski, P., Wiese, S., Sztolsztener, M. E., Oeljeklaus, S., Varabyova, A., Lirski, M., Chroscicki, P., Mroczek, S., Januszewicz, E., Dziembowski, A., Koblowska, M., Warscheid, B., & Chacinska, A. (2015). Mistargeted Mitochondrial Proteins Activate a Proteostatic Response in the Cytosol. Nature, 524(7566), 485-488. https://doi.org/10.1038/nature14951
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