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• Kreisz, P., Hellens, A.M., Fröschel, C., Krischke, M., Maag, D., Feil, R., Wildenhain, T., Draken, J., Braune, G., Erdelitsch, L., Cecchino, L., Wagner, T.C., Ache, P., Mueller, M.J., Becker, D., Lunn, J.E., Hanson, J., Beveridge, C.A., Fichtner, F., Barbier, F.F., und Weiste, C. (2024) „S(1) basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs“, Proceedings of the National Academy of Sciences of the United States of America, 121(7), e2313343121-e2313343121, verfügbar unter: https://doi.org/10.1073/pnas.2313343121.
  • [ Abstract ]
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  Plants tightly control growth of their lateral organs, which led to the concept of apical dominance. However, outgrowth of the dormant lateral primordia is sensitive to the plant's nutritional status, resulting in an immense plasticity in plant architecture. While the impact of hormonal regulation on apical dominance is well characterized, the prime importance of sugar signaling to unleash lateral organ formation has just recently emerged. Here, we aimed to identify transcriptional regulators, which control the trade-off between growth of apical versus lateral organs. Making use of locally inducible gain-of-function as well as single and higher-order loss-of-function approaches of the sugar-responsive S(1)-basic-leucine-zipper (S(1)-bZIP) transcription factors, we disclosed their largely redundant function in establishing apical growth dominance. Consistently, comprehensive phenotypical and analytical studies of S(1)-bZIP mutants show a clear shift of sugar and organic nitrogen (N) allocation from apical to lateral organs, coinciding with strong lateral organ outgrowth. Tissue-specific transcriptomics reveal specific clade III SWEET sugar transporters, crucial for long-distance sugar transport to apical sinks and the glutaminase GLUTAMINE AMIDO-TRANSFERASE 1_2.1, involved in N homeostasis, as direct S(1)-bZIP targets, linking the architectural and metabolic mutant phenotypes to downstream gene regulation. Based on these results, we propose that S(1)-bZIPs control carbohydrate (C) partitioning from source leaves to apical organs and tune systemic N supply to restrict lateral organ formation by C/N depletion. Knowledge of the underlying mechanisms controlling plant C/N partitioning is of pivotal importance for breeding strategies to generate plants with desired architectural and nutritional characteristics.

  @article{kreisz2024basic, abstract = {Plants tightly control growth of their lateral organs, which led to the concept of apical dominance. However, outgrowth of the dormant lateral primordia is sensitive to the plant's nutritional status, resulting in an immense plasticity in plant architecture. While the impact of hormonal regulation on apical dominance is well characterized, the prime importance of sugar signaling to unleash lateral organ formation has just recently emerged. Here, we aimed to identify transcriptional regulators, which control the trade-off between growth of apical versus lateral organs. Making use of locally inducible gain-of-function as well as single and higher-order loss-of-function approaches of the sugar-responsive S(1)-basic-leucine-zipper (S(1)-bZIP) transcription factors, we disclosed their largely redundant function in establishing apical growth dominance. Consistently, comprehensive phenotypical and analytical studies of S(1)-bZIP mutants show a clear shift of sugar and organic nitrogen (N) allocation from apical to lateral organs, coinciding with strong lateral organ outgrowth. Tissue-specific transcriptomics reveal specific clade III SWEET sugar transporters, crucial for long-distance sugar transport to apical sinks and the glutaminase GLUTAMINE AMIDO-TRANSFERASE 1_2.1, involved in N homeostasis, as direct S(1)-bZIP targets, linking the architectural and metabolic mutant phenotypes to downstream gene regulation. Based on these results, we propose that S(1)-bZIPs control carbohydrate (C) partitioning from source leaves to apical organs and tune systemic N supply to restrict lateral organ formation by C/N depletion. Knowledge of the underlying mechanisms controlling plant C/N partitioning is of pivotal importance for breeding strategies to generate plants with desired architectural and nutritional characteristics.}, address = {United States}, author = {Kreisz, Philipp and Hellens, Alicia M and Fröschel, Christian and Krischke, Markus and Maag, Daniel and Feil, Regina and Wildenhain, Theresa and Draken, Jan and Braune, Gabriel and Erdelitsch, Leon and Cecchino, Laura and Wagner, Tobias C and Ache, Peter and Mueller, Martin J and Becker, Dirk and Lunn, John E and Hanson, Johannes and Beveridge, Christine A and Fichtner, Franziska and Barbier, Francois F and Weiste, Christoph}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {imported}, month = {02}, number = 7, pages = {e2313343121–e2313343121}, title = {S(1) basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs}, volume = 121, year = 2024 }

  %0 Journal Article %1 kreisz2024basic %A Kreisz, Philipp %A Hellens, Alicia M %A Fröschel, Christian %A Krischke, Markus %A Maag, Daniel %A Feil, Regina %A Wildenhain, Theresa %A Draken, Jan %A Braune, Gabriel %A Erdelitsch, Leon %A Cecchino, Laura %A Wagner, Tobias C %A Ache, Peter %A Mueller, Martin J %A Becker, Dirk %A Lunn, John E %A Hanson, Johannes %A Beveridge, Christine A %A Fichtner, Franziska %A Barbier, Francois F %A Weiste, Christoph %C United States %D 2024 %J Proceedings of the National Academy of Sciences of the United States of America %N 7 %P e2313343121--e2313343121 %R 10.1073/pnas.2313343121 %T S(1) basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs %U https://pubmed.ncbi.nlm.nih.gov/38315839 %V 121 %X Plants tightly control growth of their lateral organs, which led to the concept of apical dominance. However, outgrowth of the dormant lateral primordia is sensitive to the plant's nutritional status, resulting in an immense plasticity in plant architecture. While the impact of hormonal regulation on apical dominance is well characterized, the prime importance of sugar signaling to unleash lateral organ formation has just recently emerged. Here, we aimed to identify transcriptional regulators, which control the trade-off between growth of apical versus lateral organs. Making use of locally inducible gain-of-function as well as single and higher-order loss-of-function approaches of the sugar-responsive S(1)-basic-leucine-zipper (S(1)-bZIP) transcription factors, we disclosed their largely redundant function in establishing apical growth dominance. Consistently, comprehensive phenotypical and analytical studies of S(1)-bZIP mutants show a clear shift of sugar and organic nitrogen (N) allocation from apical to lateral organs, coinciding with strong lateral organ outgrowth. Tissue-specific transcriptomics reveal specific clade III SWEET sugar transporters, crucial for long-distance sugar transport to apical sinks and the glutaminase GLUTAMINE AMIDO-TRANSFERASE 1_2.1, involved in N homeostasis, as direct S(1)-bZIP targets, linking the architectural and metabolic mutant phenotypes to downstream gene regulation. Based on these results, we propose that S(1)-bZIPs control carbohydrate (C) partitioning from source leaves to apical organs and tune systemic N supply to restrict lateral organ formation by C/N depletion. Knowledge of the underlying mechanisms controlling plant C/N partitioning is of pivotal importance for breeding strategies to generate plants with desired architectural and nutritional characteristics.

• Mueller, H.M., Franzisky, B.L., Messerer, M., Du, B., Lux, T., White, P.J., Carpentier, S.C., Winkler, J.B., Schnitzler, J.-P., El-Serehy, H.A., Al-Rasheid, K.A.S., Al-Harbi, N., Alfarraj, S., Kudla, J., Kangasjärvi, J., Reichelt, M., Mithöfer, A., Mayer, K.F.X., Rennenberg, H., Ache, P., Hedrich, R., und Geilfus, C.-M. (2023) „Integrative multi-omics analyses of date palm (Phoenix dactylifera) roots and leaves reveal how the halophyte land plant copes with sea water“, The plant genome, e20372-e20372, verfügbar unter: https://doi.org/10.1002/tpg2.20372.
  • [ Abstract ]
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  Date palm (Phoenix dactylifera L.) is able to grow and complete its life cycle while being rooted in highly saline soils. Which of the many well-known salt-tolerance strategies are combined to fine-tune this remarkable resilience is unknown. The precise location, whether in the shoot or the root, where these strategies are employed remains uncertain, leaving us unaware of how the various known salt-tolerance mechanisms are integrated to fine-tune this remarkable resilience. To address this shortcoming, we exposed date palm to a salt stress dose equivalent to seawater for up to 4 weeks and applied integrative multi-omics analyses followed by targeted metabolomics, hormone, and ion analyses. Integration of proteomic into transcriptomic data allowed a view beyond simple correlation, revealing a remarkably high degree of convergence between gene expression and protein abundance. This sheds a clear light on the acclimatization mechanisms employed, which depend on reprogramming of protein biosynthesis. For growth in highly saline habitats, date palm effectively combines various salt-tolerance mechanisms found in both halophytes and glycophytes: "avoidance" by efficient sodium and chloride exclusion at the roots, and "acclimation" by osmotic adjustment, reactive oxygen species scavenging in leaves, and remodeling of the ribosome-associated proteome in salt-exposed root cells. Combined efficiently as in P. dactylifera L., these sets of mechanisms seem to explain the palm's excellent salt stress tolerance.

  @article{mueller2023integrative, abstract = {Date palm (Phoenix dactylifera L.) is able to grow and complete its life cycle while being rooted in highly saline soils. Which of the many well-known salt-tolerance strategies are combined to fine-tune this remarkable resilience is unknown. The precise location, whether in the shoot or the root, where these strategies are employed remains uncertain, leaving us unaware of how the various known salt-tolerance mechanisms are integrated to fine-tune this remarkable resilience. To address this shortcoming, we exposed date palm to a salt stress dose equivalent to seawater for up to 4 weeks and applied integrative multi-omics analyses followed by targeted metabolomics, hormone, and ion analyses. Integration of proteomic into transcriptomic data allowed a view beyond simple correlation, revealing a remarkably high degree of convergence between gene expression and protein abundance. This sheds a clear light on the acclimatization mechanisms employed, which depend on reprogramming of protein biosynthesis. For growth in highly saline habitats, date palm effectively combines various salt-tolerance mechanisms found in both halophytes and glycophytes: "avoidance" by efficient sodium and chloride exclusion at the roots, and "acclimation" by osmotic adjustment, reactive oxygen species scavenging in leaves, and remodeling of the ribosome-associated proteome in salt-exposed root cells. Combined efficiently as in P. dactylifera L., these sets of mechanisms seem to explain the palm's excellent salt stress tolerance.}, address = {United States}, author = {Mueller, Heike M and Franzisky, Bastian L and Messerer, Maxim and Du, Baoguo and Lux, Thomas and White, Philip J and Carpentier, Sebastien Christian and Winkler, Jana Barbro and Schnitzler, Joerg-Peter and El-Serehy, Hamed A and Al-Rasheid, Khaled A S and Al-Harbi, Naif and Alfarraj, Saleh and Kudla, Jörg and Kangasjärvi, Jaakko and Reichelt, Michael and Mithöfer, Axel and Mayer, Klaus F X and Rennenberg, Heinz and Ache, Peter and Hedrich, Rainer and Geilfus, Christoph-Martin}, journal = {The plant genome}, keywords = {imported}, month = {07}, pages = {e20372–e20372}, title = {Integrative multi-omics analyses of date palm (Phoenix dactylifera) roots and leaves reveal how the halophyte land plant copes with sea water}, year = 2023 }

  %0 Journal Article %1 mueller2023integrative %A Mueller, Heike M %A Franzisky, Bastian L %A Messerer, Maxim %A Du, Baoguo %A Lux, Thomas %A White, Philip J %A Carpentier, Sebastien Christian %A Winkler, Jana Barbro %A Schnitzler, Joerg-Peter %A El-Serehy, Hamed A %A Al-Rasheid, Khaled A S %A Al-Harbi, Naif %A Alfarraj, Saleh %A Kudla, Jörg %A Kangasjärvi, Jaakko %A Reichelt, Michael %A Mithöfer, Axel %A Mayer, Klaus F X %A Rennenberg, Heinz %A Ache, Peter %A Hedrich, Rainer %A Geilfus, Christoph-Martin %C United States %D 2023 %J The plant genome %P e20372--e20372 %R 10.1002/tpg2.20372 %T Integrative multi-omics analyses of date palm (Phoenix dactylifera) roots and leaves reveal how the halophyte land plant copes with sea water %U https://pubmed.ncbi.nlm.nih.gov/37518859 %X Date palm (Phoenix dactylifera L.) is able to grow and complete its life cycle while being rooted in highly saline soils. Which of the many well-known salt-tolerance strategies are combined to fine-tune this remarkable resilience is unknown. The precise location, whether in the shoot or the root, where these strategies are employed remains uncertain, leaving us unaware of how the various known salt-tolerance mechanisms are integrated to fine-tune this remarkable resilience. To address this shortcoming, we exposed date palm to a salt stress dose equivalent to seawater for up to 4 weeks and applied integrative multi-omics analyses followed by targeted metabolomics, hormone, and ion analyses. Integration of proteomic into transcriptomic data allowed a view beyond simple correlation, revealing a remarkably high degree of convergence between gene expression and protein abundance. This sheds a clear light on the acclimatization mechanisms employed, which depend on reprogramming of protein biosynthesis. For growth in highly saline habitats, date palm effectively combines various salt-tolerance mechanisms found in both halophytes and glycophytes: "avoidance" by efficient sodium and chloride exclusion at the roots, and "acclimation" by osmotic adjustment, reactive oxygen species scavenging in leaves, and remodeling of the ribosome-associated proteome in salt-exposed root cells. Combined efficiently as in P. dactylifera L., these sets of mechanisms seem to explain the palm's excellent salt stress tolerance.
• Du, B., Winkler, J.B., Ache, P., White, P.J., Dannenmann, M., Alfarraj, S., Albasher, G., Schnitzler, J.-P., Hedrich, R., und Rennenberg, H. (2023) „Differences of nitrogen metabolism in date palm (Phoenix dactylifera) seedlings subjected to water deprivation and salt exposure“, Tree physiology, 43(4), 587–596, verfügbar unter: https://doi.org/10.1093/treephys/tpac145.
  • [ Abstract ]
  • [ BibTeX ]
  • [ EndNote ]
  • [ URL ]
  • [ DOI ]
  Drought and salt exposure are among the most prevalent and severe abiotic stressors causing serious agricultural yield losses, alone and in combination. Little is known about differences and similarities in the effects of these two stress factors on plant metabolic regulation, particularly on nitrogen metabolism. Here, we studied the effects of water deprivation and salt exposure on water relations and nitrogen metabolites in leaves and roots of date palm seedlings. Both, water deprivation and salt exposure had no significant effects on plant water content or stable carbon (C) and nitrogen (N) isotope signatures. Significant effects of water deprivation on total C and N concentrations were only observed in roots, i.e., decreased total C and increased total N concentrations. Whereas salt exposure initially decreased total C and increased total N concentrations significantly in roots, foliar total C concentration was increased upon prolonged exposure. Initially C/N ratios declined in roots of plants from both treatments and upon prolonged salt exposure also in the leaves. Neither treatment affected soluble protein and structural N concentrations in leaves or roots, but resulted in the accumulation of most amino acids, except for glutamate and tryptophan, which remained stable, and serine, which decreased, in roots. Accumulation of the most abundant amino acids, lysine and proline, was observed in roots under both treatments, but in leaves only upon salt exposure. This finding indicates a similar role of these amino acids as compatible solutes in the roots in response to salt und drought, but not in the leaves. Upon prolonged treatment, amino acid concentrations returned to levels found in unstressed plants in leaves of water deprived, but not salt exposed, plants. The present results show both water deprivation and salt exposure strongly impact N metabolism of date palm seedlings, but in a different manner in leaves and roots.

  @article{du2023differences, abstract = {Drought and salt exposure are among the most prevalent and severe abiotic stressors causing serious agricultural yield losses, alone and in combination. Little is known about differences and similarities in the effects of these two stress factors on plant metabolic regulation, particularly on nitrogen metabolism. Here, we studied the effects of water deprivation and salt exposure on water relations and nitrogen metabolites in leaves and roots of date palm seedlings. Both, water deprivation and salt exposure had no significant effects on plant water content or stable carbon (C) and nitrogen (N) isotope signatures. Significant effects of water deprivation on total C and N concentrations were only observed in roots, i.e., decreased total C and increased total N concentrations. Whereas salt exposure initially decreased total C and increased total N concentrations significantly in roots, foliar total C concentration was increased upon prolonged exposure. Initially C/N ratios declined in roots of plants from both treatments and upon prolonged salt exposure also in the leaves. Neither treatment affected soluble protein and structural N concentrations in leaves or roots, but resulted in the accumulation of most amino acids, except for glutamate and tryptophan, which remained stable, and serine, which decreased, in roots. Accumulation of the most abundant amino acids, lysine and proline, was observed in roots under both treatments, but in leaves only upon salt exposure. This finding indicates a similar role of these amino acids as compatible solutes in the roots in response to salt und drought, but not in the leaves. Upon prolonged treatment, amino acid concentrations returned to levels found in unstressed plants in leaves of water deprived, but not salt exposed, plants. The present results show both water deprivation and salt exposure strongly impact N metabolism of date palm seedlings, but in a different manner in leaves and roots.}, address = {Canada}, author = {Du, Baoguo and Winkler, Jana Barbro and Ache, Peter and White, Philip J and Dannenmann, Michael and Alfarraj, Saleh and Albasher, Gadah and Schnitzler, Joerg-Peter and Hedrich, Rainer and Rennenberg, Heinz}, journal = {Tree physiology}, keywords = {imported}, month = {04}, number = 4, pages = {587–596}, title = {Differences of nitrogen metabolism in date palm (Phoenix dactylifera) seedlings subjected to water deprivation and salt exposure}, volume = 43, year = 2023 }

  %0 Journal Article %1 du2023differences %A Du, Baoguo %A Winkler, Jana Barbro %A Ache, Peter %A White, Philip J %A Dannenmann, Michael %A Alfarraj, Saleh %A Albasher, Gadah %A Schnitzler, Joerg-Peter %A Hedrich, Rainer %A Rennenberg, Heinz %C Canada %D 2023 %J Tree physiology %N 4 %P 587--596 %R 10.1093/treephys/tpac145 %T Differences of nitrogen metabolism in date palm (Phoenix dactylifera) seedlings subjected to water deprivation and salt exposure %U https://pubmed.ncbi.nlm.nih.gov/36579827 %V 43 %X Drought and salt exposure are among the most prevalent and severe abiotic stressors causing serious agricultural yield losses, alone and in combination. Little is known about differences and similarities in the effects of these two stress factors on plant metabolic regulation, particularly on nitrogen metabolism. Here, we studied the effects of water deprivation and salt exposure on water relations and nitrogen metabolites in leaves and roots of date palm seedlings. Both, water deprivation and salt exposure had no significant effects on plant water content or stable carbon (C) and nitrogen (N) isotope signatures. Significant effects of water deprivation on total C and N concentrations were only observed in roots, i.e., decreased total C and increased total N concentrations. Whereas salt exposure initially decreased total C and increased total N concentrations significantly in roots, foliar total C concentration was increased upon prolonged exposure. Initially C/N ratios declined in roots of plants from both treatments and upon prolonged salt exposure also in the leaves. Neither treatment affected soluble protein and structural N concentrations in leaves or roots, but resulted in the accumulation of most amino acids, except for glutamate and tryptophan, which remained stable, and serine, which decreased, in roots. Accumulation of the most abundant amino acids, lysine and proline, was observed in roots under both treatments, but in leaves only upon salt exposure. This finding indicates a similar role of these amino acids as compatible solutes in the roots in response to salt und drought, but not in the leaves. Upon prolonged treatment, amino acid concentrations returned to levels found in unstressed plants in leaves of water deprived, but not salt exposed, plants. The present results show both water deprivation and salt exposure strongly impact N metabolism of date palm seedlings, but in a different manner in leaves and roots.
• Arab, L., Hoshika, Y., Paoletti, E., White, P.J., Dannenmann, M., Mueller, H., Ache, P., Hedrich, R., Alfarraj, S., Albasher, G., und Rennenberg, H. (2023) „Chronic ozone exposure impairs the mineral nutrition of date palm (Phoenix dactylifera) seedlings“, The Science of the total environment, 862, 160675–160675, verfügbar unter: https://doi.org/10.1016/j.scitotenv.2022.160675.
  • [ Abstract ]
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  Chronic ozone (O(3)) exposure in the atmosphere preferentially disturbs metabolic processes in the roots rather than the shoot as a consequence of reduced photosynthesis and carbohydrate allocation from the leaves to the roots. The aim of the present study was to elucidate if mineral nutrition is also impaired by chronic O(3) exposure. For this purpose, date palm (Phoenix dactylifera) plants were fumigated with ambient, 1.5 × ambient and 2 × ambient O(3) in a free air controlled exposure (FACE) system for one growing season and concentrations of major nutrients were analyzed in leaves and roots. In addition, concentrations of C and N and their partitioning between different metabolic C and N pools were determined in both organs. The results showed that calcium (Ca), magnesium (Mg), iron (Fe), zinc (Zn), sodium (Na) and potassium (K) acquisition by roots was diminished by O(3) exposure of the shoot. For Ca, Mg, Fe and Zn reduced uptake by the roots was combined with reduced allocation to the shoot, resulting in a decline of foliar concentrations; for Na and K, allocation to the shoot was maintained at the expense of the roots. Thus, elevated O(3) impaired both mineral uptake by the roots and partitioning of minerals between roots and shoots, but in an element specific way. Thereby, elevated O(3) affected roots and shoots differently already after one growing season. However, considerable changes in total C and N concentrations and their partitioning between different metabolic pools upon chronic O(3) exposure were not observed in either leaves or roots, except for reduced foliar lignin concentrations at 2 × ambient O(3). Significant differences in these parameters were shown between leaves and roots independent of O(3) application. The physiological consequences of the effects of chronic O(3) exposure on mineral acquisition and partitioning between leaves and roots are discussed.

  @article{arab2023chronic, abstract = {Chronic ozone (O(3)) exposure in the atmosphere preferentially disturbs metabolic processes in the roots rather than the shoot as a consequence of reduced photosynthesis and carbohydrate allocation from the leaves to the roots. The aim of the present study was to elucidate if mineral nutrition is also impaired by chronic O(3) exposure. For this purpose, date palm (Phoenix dactylifera) plants were fumigated with ambient, 1.5 × ambient and 2 × ambient O(3) in a free air controlled exposure (FACE) system for one growing season and concentrations of major nutrients were analyzed in leaves and roots. In addition, concentrations of C and N and their partitioning between different metabolic C and N pools were determined in both organs. The results showed that calcium (Ca), magnesium (Mg), iron (Fe), zinc (Zn), sodium (Na) and potassium (K) acquisition by roots was diminished by O(3) exposure of the shoot. For Ca, Mg, Fe and Zn reduced uptake by the roots was combined with reduced allocation to the shoot, resulting in a decline of foliar concentrations; for Na and K, allocation to the shoot was maintained at the expense of the roots. Thus, elevated O(3) impaired both mineral uptake by the roots and partitioning of minerals between roots and shoots, but in an element specific way. Thereby, elevated O(3) affected roots and shoots differently already after one growing season. However, considerable changes in total C and N concentrations and their partitioning between different metabolic pools upon chronic O(3) exposure were not observed in either leaves or roots, except for reduced foliar lignin concentrations at 2 × ambient O(3). Significant differences in these parameters were shown between leaves and roots independent of O(3) application. The physiological consequences of the effects of chronic O(3) exposure on mineral acquisition and partitioning between leaves and roots are discussed.}, address = {Netherlands}, author = {Arab, Leila and Hoshika, Yasutomo and Paoletti, Elena and White, Philip J and Dannenmann, Michael and Mueller, Heike and Ache, Peter and Hedrich, Rainer and Alfarraj, Saleh and Albasher, Ghada and Rennenberg, Heinz}, journal = {The Science of the total environment}, keywords = {imported}, month = {03}, pages = {160675–160675}, title = {Chronic ozone exposure impairs the mineral nutrition of date palm (Phoenix dactylifera) seedlings}, volume = 862, year = 2023 }

  %0 Journal Article %1 arab2023chronic %A Arab, Leila %A Hoshika, Yasutomo %A Paoletti, Elena %A White, Philip J %A Dannenmann, Michael %A Mueller, Heike %A Ache, Peter %A Hedrich, Rainer %A Alfarraj, Saleh %A Albasher, Ghada %A Rennenberg, Heinz %C Netherlands %D 2023 %J The Science of the total environment %P 160675--160675 %R 10.1016/j.scitotenv.2022.160675 %T Chronic ozone exposure impairs the mineral nutrition of date palm (Phoenix dactylifera) seedlings %U https://pubmed.ncbi.nlm.nih.gov/36481139 %V 862 %X Chronic ozone (O(3)) exposure in the atmosphere preferentially disturbs metabolic processes in the roots rather than the shoot as a consequence of reduced photosynthesis and carbohydrate allocation from the leaves to the roots. The aim of the present study was to elucidate if mineral nutrition is also impaired by chronic O(3) exposure. For this purpose, date palm (Phoenix dactylifera) plants were fumigated with ambient, 1.5 × ambient and 2 × ambient O(3) in a free air controlled exposure (FACE) system for one growing season and concentrations of major nutrients were analyzed in leaves and roots. In addition, concentrations of C and N and their partitioning between different metabolic C and N pools were determined in both organs. The results showed that calcium (Ca), magnesium (Mg), iron (Fe), zinc (Zn), sodium (Na) and potassium (K) acquisition by roots was diminished by O(3) exposure of the shoot. For Ca, Mg, Fe and Zn reduced uptake by the roots was combined with reduced allocation to the shoot, resulting in a decline of foliar concentrations; for Na and K, allocation to the shoot was maintained at the expense of the roots. Thus, elevated O(3) impaired both mineral uptake by the roots and partitioning of minerals between roots and shoots, but in an element specific way. Thereby, elevated O(3) affected roots and shoots differently already after one growing season. However, considerable changes in total C and N concentrations and their partitioning between different metabolic pools upon chronic O(3) exposure were not observed in either leaves or roots, except for reduced foliar lignin concentrations at 2 × ambient O(3). Significant differences in these parameters were shown between leaves and roots independent of O(3) application. The physiological consequences of the effects of chronic O(3) exposure on mineral acquisition and partitioning between leaves and roots are discussed.

• Bazihizina, N., Böhm, J., Messerer, M., Stigloher, C., Müller, H.M., Cuin, T.A., Maierhofer, T., Cabot, J., Mayer, K.F.X., Fella, C., Huang, S., Al-Rasheid, K.A.S., Alquraishi, S., Breadmore, M., Mancuso, S., Shabala, S., Ache, P., Zhang, H., Zhu, J.-K., Hedrich, R., und Scherzer, S. (2022) „Stalk cell polar ion transport provide for bladder-based salinity tolerance in Chenopodium quinoa“, The New phytologist, 235(5), 1822–1835, verfügbar unter: https://doi.org/10.1111/nph.18205.
  • [ Abstract ]
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  Chenopodium quinoa uses epidermal bladder cells (EBCs) to sequester excess salt. Each EBC complex consists of a leaf epidermal cell, a stalk cell, and the bladder. Under salt stress, sodium (Na(+) ), chloride (Cl(-) ), potassium (K(+) ) and various metabolites are shuttled from the leaf lamina to the bladders. Stalk cells operate as both a selectivity filter and a flux controller. In line with the nature of a transfer cell, advanced transmission electron tomography, electrophysiology, and fluorescent tracer flux studies revealed the stalk cell's polar organization and bladder-directed solute flow. RNA sequencing and cluster analysis revealed the gene expression profiles of the stalk cells. Among the stalk cell enriched genes, ion channels and carriers as well as sugar transporters were most pronounced. Based on their electrophysiological fingerprint and thermodynamic considerations, a model for stalk cell transcellular transport was derived.

  @article{bazihizina2022stalk, abstract = {Chenopodium quinoa uses epidermal bladder cells (EBCs) to sequester excess salt. Each EBC complex consists of a leaf epidermal cell, a stalk cell, and the bladder. Under salt stress, sodium (Na(+) ), chloride (Cl(-) ), potassium (K(+) ) and various metabolites are shuttled from the leaf lamina to the bladders. Stalk cells operate as both a selectivity filter and a flux controller. In line with the nature of a transfer cell, advanced transmission electron tomography, electrophysiology, and fluorescent tracer flux studies revealed the stalk cell's polar organization and bladder-directed solute flow. RNA sequencing and cluster analysis revealed the gene expression profiles of the stalk cells. Among the stalk cell enriched genes, ion channels and carriers as well as sugar transporters were most pronounced. Based on their electrophysiological fingerprint and thermodynamic considerations, a model for stalk cell transcellular transport was derived.}, address = {England}, author = {Bazihizina, Nadia and Böhm, Jennifer and Messerer, Maxim and Stigloher, Christian and Müller, Heike M and Cuin, Tracey Ann and Maierhofer, Tobias and Cabot, Joan and Mayer, Klaus F X and Fella, Christian and Huang, Shouguang and Al-Rasheid, Khaled A S and Alquraishi, Saleh and Breadmore, Michael and Mancuso, Stefano and Shabala, Sergey and Ache, Peter and Zhang, Heng and Zhu, Jian-Kang and Hedrich, Rainer and Scherzer, Sönke}, journal = {The New phytologist}, keywords = {imported}, month = {09}, number = 5, pages = {1822–1835}, title = {Stalk cell polar ion transport provide for bladder-based salinity tolerance in Chenopodium quinoa}, volume = 235, year = 2022 }

  %0 Journal Article %1 bazihizina2022stalk %A Bazihizina, Nadia %A Böhm, Jennifer %A Messerer, Maxim %A Stigloher, Christian %A Müller, Heike M %A Cuin, Tracey Ann %A Maierhofer, Tobias %A Cabot, Joan %A Mayer, Klaus F X %A Fella, Christian %A Huang, Shouguang %A Al-Rasheid, Khaled A S %A Alquraishi, Saleh %A Breadmore, Michael %A Mancuso, Stefano %A Shabala, Sergey %A Ache, Peter %A Zhang, Heng %A Zhu, Jian-Kang %A Hedrich, Rainer %A Scherzer, Sönke %C England %D 2022 %J The New phytologist %N 5 %P 1822--1835 %R 10.1111/nph.18205 %T Stalk cell polar ion transport provide for bladder-based salinity tolerance in Chenopodium quinoa %U https://pubmed.ncbi.nlm.nih.gov/35510810 %V 235 %X Chenopodium quinoa uses epidermal bladder cells (EBCs) to sequester excess salt. Each EBC complex consists of a leaf epidermal cell, a stalk cell, and the bladder. Under salt stress, sodium (Na(+) ), chloride (Cl(-) ), potassium (K(+) ) and various metabolites are shuttled from the leaf lamina to the bladders. Stalk cells operate as both a selectivity filter and a flux controller. In line with the nature of a transfer cell, advanced transmission electron tomography, electrophysiology, and fluorescent tracer flux studies revealed the stalk cell's polar organization and bladder-directed solute flow. RNA sequencing and cluster analysis revealed the gene expression profiles of the stalk cells. Among the stalk cell enriched genes, ion channels and carriers as well as sugar transporters were most pronounced. Based on their electrophysiological fingerprint and thermodynamic considerations, a model for stalk cell transcellular transport was derived.
• Arab, L., Hoshika, Y., Müller, H., Cotrozzi, L., Nali, C., Tonelli, M., Ache, P., Paoletti, E., Alfarraj, S., Albasher, G., Hedrich, R., und Rennenberg, H. (2022) „Chronic ozone exposure preferentially modifies root rather than foliar metabolism of date palm (Phoenix dactylifera) saplings“, The Science of the total environment, 806(Pt 2), 150563–150563, verfügbar unter: https://doi.org/10.1016/j.scitotenv.2021.150563.
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  In their natural environment, date palms are exposed to chronic atmospheric ozone (O(3)) concentrations from local and remote sources. In order to elucidate the consequences of this exposure, date palm saplings were treated with ambient, 1.5 and 2.0 times ambient O(3) for three months in a free-air controlled exposure facility. Chronic O(3) exposure reduced carbohydrate contents in leaves and roots, but this effect was much stronger in roots. Still, sucrose contents of both organs were maintained at elevated O(3), though at different steady states. Reduced availability of carbohydrate for the Tricarboxylic acid cycle (TCA cycle) may be responsible for the observed reduced foliar contents of several amino acids, whereas malic acid accumulation in the roots indicates a reduced use of TCA cycle intermediates. Carbohydrate deficiency in roots, but not in leaves caused oxidative stress upon chronic O(3) exposure, as indicated by enhanced malonedialdehyde, H(2)O(2) and oxidized glutathione contents despite elevated glutathione reductase activity. Reduced levels of phenolics and flavonoids in the roots resulted from decreased production and, therefore, do not indicate oxidative stress compensation by secondary compounds. These results show that roots of date palms are highly susceptible to chronic O(3) exposure as a consequence of carbohydrate deficiency.

  @article{arab2022chronic, abstract = {In their natural environment, date palms are exposed to chronic atmospheric ozone (O(3)) concentrations from local and remote sources. In order to elucidate the consequences of this exposure, date palm saplings were treated with ambient, 1.5 and 2.0 times ambient O(3) for three months in a free-air controlled exposure facility. Chronic O(3) exposure reduced carbohydrate contents in leaves and roots, but this effect was much stronger in roots. Still, sucrose contents of both organs were maintained at elevated O(3), though at different steady states. Reduced availability of carbohydrate for the Tricarboxylic acid cycle (TCA cycle) may be responsible for the observed reduced foliar contents of several amino acids, whereas malic acid accumulation in the roots indicates a reduced use of TCA cycle intermediates. Carbohydrate deficiency in roots, but not in leaves caused oxidative stress upon chronic O(3) exposure, as indicated by enhanced malonedialdehyde, H(2)O(2) and oxidized glutathione contents despite elevated glutathione reductase activity. Reduced levels of phenolics and flavonoids in the roots resulted from decreased production and, therefore, do not indicate oxidative stress compensation by secondary compounds. These results show that roots of date palms are highly susceptible to chronic O(3) exposure as a consequence of carbohydrate deficiency.}, address = {Netherlands}, author = {Arab, L and Hoshika, Y and Müller, H and Cotrozzi, L and Nali, C and Tonelli, M and Ache, P and Paoletti, E and Alfarraj, S and Albasher, G and Hedrich, R and Rennenberg, H}, journal = {The Science of the total environment}, keywords = {imported}, month = {02}, number = {Pt 2}, pages = {150563–150563}, title = {Chronic ozone exposure preferentially modifies root rather than foliar metabolism of date palm (Phoenix dactylifera) saplings}, volume = 806, year = 2022 }

  %0 Journal Article %1 arab2022chronic %A Arab, L %A Hoshika, Y %A Müller, H %A Cotrozzi, L %A Nali, C %A Tonelli, M %A Ache, P %A Paoletti, E %A Alfarraj, S %A Albasher, G %A Hedrich, R %A Rennenberg, H %C Netherlands %D 2022 %J The Science of the total environment %N Pt 2 %P 150563--150563 %R 10.1016/j.scitotenv.2021.150563 %T Chronic ozone exposure preferentially modifies root rather than foliar metabolism of date palm (Phoenix dactylifera) saplings %U https://pubmed.ncbi.nlm.nih.gov/34601178 %V 806 %X In their natural environment, date palms are exposed to chronic atmospheric ozone (O(3)) concentrations from local and remote sources. In order to elucidate the consequences of this exposure, date palm saplings were treated with ambient, 1.5 and 2.0 times ambient O(3) for three months in a free-air controlled exposure facility. Chronic O(3) exposure reduced carbohydrate contents in leaves and roots, but this effect was much stronger in roots. Still, sucrose contents of both organs were maintained at elevated O(3), though at different steady states. Reduced availability of carbohydrate for the Tricarboxylic acid cycle (TCA cycle) may be responsible for the observed reduced foliar contents of several amino acids, whereas malic acid accumulation in the roots indicates a reduced use of TCA cycle intermediates. Carbohydrate deficiency in roots, but not in leaves caused oxidative stress upon chronic O(3) exposure, as indicated by enhanced malonedialdehyde, H(2)O(2) and oxidized glutathione contents despite elevated glutathione reductase activity. Reduced levels of phenolics and flavonoids in the roots resulted from decreased production and, therefore, do not indicate oxidative stress compensation by secondary compounds. These results show that roots of date palms are highly susceptible to chronic O(3) exposure as a consequence of carbohydrate deficiency.

• Ghirardo, A., Nosenko, T., Kreuzwieser, J., Winkler, J.B., Kruse, J., Albert, A., Merl-Pham, J., Lux, T., Ache, P., Zimmer, I., Alfarraj, S., Mayer, K.F.X., Hedrich, R., Rennenberg, H., und Schnitzler, J.-P. (2021) „Protein expression plasticity contributes to heat and drought tolerance of date palm“, Oecologia, 197(4), 903–919, verfügbar unter: https://doi.org/10.1007/s00442-021-04907-w.
  • [ Abstract ]
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  Climate change is increasing the frequency and intensity of warming and drought periods around the globe, currently representing a threat to many plant species. Understanding the resistance and resilience of plants to climate change is, therefore, urgently needed. As date palm (Phoenix dactylifera) evolved adaptation mechanisms to a xeric environment and can tolerate large diurnal and seasonal temperature fluctuations, we studied the protein expression changes in leaves, volatile organic compound emissions, and photosynthesis in response to variable growth temperatures and soil water deprivation. Plants were grown under controlled environmental conditions of simulated Saudi Arabian summer and winter climates challenged with drought stress. We show that date palm is able to counteract the harsh conditions of the Arabian Peninsula by adjusting the abundances of proteins related to the photosynthetic machinery, abiotic stress and secondary metabolism. Under summer climate and water deprivation, these adjustments included efficient protein expression response mediated by heat shock proteins and the antioxidant system to counteract reactive oxygen species formation. Proteins related to secondary metabolism were downregulated, except for the P. dactylifera isoprene synthase (PdIspS), which was strongly upregulated in response to summer climate and drought. This study reports, for the first time, the identification and functional characterization of the gene encoding for PdIspS, allowing future analysis of isoprene functions in date palm under extreme environments. Overall, the current study shows that reprogramming of the leaf protein profiles confers the date palm heat- and drought tolerance. We conclude that the protein plasticity of date palm is an important mechanism of molecular adaptation to environmental fluctuations.

  @article{ghirardo2021protein, abstract = {Climate change is increasing the frequency and intensity of warming and drought periods around the globe, currently representing a threat to many plant species. Understanding the resistance and resilience of plants to climate change is, therefore, urgently needed. As date palm (Phoenix dactylifera) evolved adaptation mechanisms to a xeric environment and can tolerate large diurnal and seasonal temperature fluctuations, we studied the protein expression changes in leaves, volatile organic compound emissions, and photosynthesis in response to variable growth temperatures and soil water deprivation. Plants were grown under controlled environmental conditions of simulated Saudi Arabian summer and winter climates challenged with drought stress. We show that date palm is able to counteract the harsh conditions of the Arabian Peninsula by adjusting the abundances of proteins related to the photosynthetic machinery, abiotic stress and secondary metabolism. Under summer climate and water deprivation, these adjustments included efficient protein expression response mediated by heat shock proteins and the antioxidant system to counteract reactive oxygen species formation. Proteins related to secondary metabolism were downregulated, except for the P. dactylifera isoprene synthase (PdIspS), which was strongly upregulated in response to summer climate and drought. This study reports, for the first time, the identification and functional characterization of the gene encoding for PdIspS, allowing future analysis of isoprene functions in date palm under extreme environments. Overall, the current study shows that reprogramming of the leaf protein profiles confers the date palm heat- and drought tolerance. We conclude that the protein plasticity of date palm is an important mechanism of molecular adaptation to environmental fluctuations.}, address = {Germany}, author = {Ghirardo, Andrea and Nosenko, Tetyana and Kreuzwieser, Jürgen and Winkler, J Barbro and Kruse, Jörg and Albert, Andreas and Merl-Pham, Juliane and Lux, Thomas and Ache, Peter and Zimmer, Ina and Alfarraj, Saleh and Mayer, Klaus F X and Hedrich, Rainer and Rennenberg, Heinz and Schnitzler, Jörg-Peter}, journal = {Oecologia}, keywords = {imported}, month = 12, number = 4, pages = {903–919}, title = {Protein expression plasticity contributes to heat and drought tolerance of date palm}, volume = 197, year = 2021 }

  %0 Journal Article %1 ghirardo2021protein %A Ghirardo, Andrea %A Nosenko, Tetyana %A Kreuzwieser, Jürgen %A Winkler, J Barbro %A Kruse, Jörg %A Albert, Andreas %A Merl-Pham, Juliane %A Lux, Thomas %A Ache, Peter %A Zimmer, Ina %A Alfarraj, Saleh %A Mayer, Klaus F X %A Hedrich, Rainer %A Rennenberg, Heinz %A Schnitzler, Jörg-Peter %C Germany %D 2021 %J Oecologia %N 4 %P 903--919 %R 10.1007/s00442-021-04907-w %T Protein expression plasticity contributes to heat and drought tolerance of date palm %U https://pubmed.ncbi.nlm.nih.gov/33880635 %V 197 %X Climate change is increasing the frequency and intensity of warming and drought periods around the globe, currently representing a threat to many plant species. Understanding the resistance and resilience of plants to climate change is, therefore, urgently needed. As date palm (Phoenix dactylifera) evolved adaptation mechanisms to a xeric environment and can tolerate large diurnal and seasonal temperature fluctuations, we studied the protein expression changes in leaves, volatile organic compound emissions, and photosynthesis in response to variable growth temperatures and soil water deprivation. Plants were grown under controlled environmental conditions of simulated Saudi Arabian summer and winter climates challenged with drought stress. We show that date palm is able to counteract the harsh conditions of the Arabian Peninsula by adjusting the abundances of proteins related to the photosynthetic machinery, abiotic stress and secondary metabolism. Under summer climate and water deprivation, these adjustments included efficient protein expression response mediated by heat shock proteins and the antioxidant system to counteract reactive oxygen species formation. Proteins related to secondary metabolism were downregulated, except for the P. dactylifera isoprene synthase (PdIspS), which was strongly upregulated in response to summer climate and drought. This study reports, for the first time, the identification and functional characterization of the gene encoding for PdIspS, allowing future analysis of isoprene functions in date palm under extreme environments. Overall, the current study shows that reprogramming of the leaf protein profiles confers the date palm heat- and drought tolerance. We conclude that the protein plasticity of date palm is an important mechanism of molecular adaptation to environmental fluctuations.
• Garcia-Maquilon, I., Coego, A., Lozano-Juste, J., Messerer, M., de Ollas, C., Julian, J., Ruiz-Partida, R., Pizzio, G., Belda-Palaz{{ó}}n, B., Gomez-Cadenas, A., Mayer, K.F.X., Geiger, D., Alquraishi, S.A., Alrefaei, A.F., Ache, P., Hedrich, R., und Rodriguez, P.L. (2021) „{PYL}8 {ABA} receptors of Phoenix dactylifera play a crucial role in response to abiotic stress and are stabilized by {ABA}“, Journal of Experimental Botany, 72(2), 757–774, verfügbar unter: https://doi.org/10.1093/jxb/eraa476.
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  @article{Garcia_Maquilon_2020, author = {Garcia-Maquilon, Irene and Coego, Alberto and Lozano-Juste, Jorge and Messerer, Maxim and de Ollas, Carlos and Julian, Jose and Ruiz-Partida, Rafael and Pizzio, Gaston and Belda-Palaz{\'{o}}n, Borja and Gomez-Cadenas, Aurelio and Mayer, Klaus F X and Geiger, Dietmar and Alquraishi, Saleh A and Alrefaei, Abdulwahed F and Ache, Peter and Hedrich, Rainer and Rodriguez, Pedro L}, editor = {Jones, Matt}, journal = {Journal of Experimental Botany}, keywords = {myown}, month = 10, number = 2, pages = {757-774}, publisher = {Oxford University Press ({OUP})}, title = {{PYL}8 {ABA} receptors of Phoenix dactylifera play a crucial role in response to abiotic stress and are stabilized by {ABA}}, volume = 72, year = 2021 }

  %0 Journal Article %1 Garcia_Maquilon_2020 %A Garcia-Maquilon, Irene %A Coego, Alberto %A Lozano-Juste, Jorge %A Messerer, Maxim %A de Ollas, Carlos %A Julian, Jose %A Ruiz-Partida, Rafael %A Pizzio, Gaston %A Belda-Palaz{{ó}}n, Borja %A Gomez-Cadenas, Aurelio %A Mayer, Klaus F X %A Geiger, Dietmar %A Alquraishi, Saleh A %A Alrefaei, Abdulwahed F %A Ache, Peter %A Hedrich, Rainer %A Rodriguez, Pedro L %D 2021 %E Jones, Matt %I Oxford University Press ({OUP}) %J Journal of Experimental Botany %N 2 %P 757-774 %R 10.1093/jxb/eraa476 %T {PYL}8 {ABA} receptors of Phoenix dactylifera play a crucial role in response to abiotic stress and are stabilized by {ABA} %V 72
• Paoletti, E., Hoshika, Y., Arab, L., Martini, S., Cotrozzi, L., Weber, D., Ache, P., Neri, L., Baraldi, R., Pellegrini, E., Müller, H.M., Hedrich, R., Alfarraj, S., und Rennenberg, H. (2021) „Date palm responses to a chronic, realistic ozone exposure in a {FACE} experiment“, Environmental Research, 195, 110868, verfügbar unter: https://doi.org/10.1016/j.envres.2021.110868.
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  @article{Paoletti_2021, author = {Paoletti, Elena and Hoshika, Yasutomo and Arab, Leila and Martini, Sofia and Cotrozzi, Lorenzo and Weber, Daniel and Ache, Peter and Neri, Luisa and Baraldi, Rita and Pellegrini, Elisa and Müller, Heike M. and Hedrich, Rainer and Alfarraj, Saleh and Rennenberg, Heinz}, journal = {Environmental Research}, keywords = {imported}, month = {04}, pages = 110868, publisher = {Elsevier {BV}}, title = {Date palm responses to a chronic, realistic ozone exposure in a {FACE} experiment}, volume = 195, year = 2021 }

  %0 Journal Article %1 Paoletti_2021 %A Paoletti, Elena %A Hoshika, Yasutomo %A Arab, Leila %A Martini, Sofia %A Cotrozzi, Lorenzo %A Weber, Daniel %A Ache, Peter %A Neri, Luisa %A Baraldi, Rita %A Pellegrini, Elisa %A Müller, Heike M. %A Hedrich, Rainer %A Alfarraj, Saleh %A Rennenberg, Heinz %D 2021 %I Elsevier {BV} %J Environmental Research %P 110868 %R 10.1016/j.envres.2021.110868 %T Date palm responses to a chronic, realistic ozone exposure in a {FACE} experiment %V 195
• Karimi, S.M., Freund, M., Wager, B.M., Knoblauch, M., Fromm, J., Müller, H., Ache, P., Krischke, M., Mueller, M.J., Müller, T., Dittrich, M., Geilfus, C.-M., Alfarhan, A.H., Hedrich, R., und Deeken, R. (2021) „Under salt stress guard cells rewire ion transport and abscisic acid ({ABA}) signaling“, New Phytologist, verfügbar unter: https://doi.org/10.1111/nph.17376.
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  @article{Karimi_2021, author = {Karimi, Sohail M. and Freund, Matthias and Wager, Brittney M. and Knoblauch, Michael and Fromm, Jörg and Müller, Heike and Ache, Peter and Krischke, Markus and Mueller, Martin J. and Müller, Tobias and Dittrich, Marcus and Geilfus, Christoph-Martin and Alfarhan, Ahmed H. and Hedrich, Rainer and Deeken, Rosalia}, journal = {New Phytologist}, keywords = {imported}, month = {03}, publisher = {Wiley}, title = {Under salt stress guard cells rewire ion transport and abscisic acid ({ABA}) signaling}, year = 2021 }

  %0 Journal Article %1 Karimi_2021 %A Karimi, Sohail M. %A Freund, Matthias %A Wager, Brittney M. %A Knoblauch, Michael %A Fromm, Jörg %A Müller, Heike %A Ache, Peter %A Krischke, Markus %A Mueller, Martin J. %A Müller, Tobias %A Dittrich, Marcus %A Geilfus, Christoph-Martin %A Alfarhan, Ahmed H. %A Hedrich, Rainer %A Deeken, Rosalia %D 2021 %I Wiley %J New Phytologist %R 10.1111/nph.17376 %T Under salt stress guard cells rewire ion transport and abscisic acid ({ABA}) signaling
• Du, B., Kruse, J., Winkler, J.B., Alfarraj, S., Albasher, G., Schnitzler, J.-P., Ache, P., Hedrich, R., und Rennenberg, H. (2021) „Metabolic responses of date palm (Phoenix dactylifera L.) leaves to drought differ in summer and winter climate“, Tree Physiology, verfügbar unter: https://doi.org/10.1093/treephys/tpab027.
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  @article{Du_2021, author = {Du, Baoguo and Kruse, Joerg and Winkler, Jana Barbro and Alfarraj, Saleh and Albasher, Gadah and Schnitzler, Joerg-Peter and Ache, Peter and Hedrich, Rainer and Rennenberg, Heinz}, journal = {Tree Physiology}, keywords = {myown}, month = {02}, publisher = {Oxford University Press ({OUP})}, title = {Metabolic responses of date palm (Phoenix dactylifera L.) leaves to drought differ in summer and winter climate}, year = 2021 }

  %0 Journal Article %1 Du_2021 %A Du, Baoguo %A Kruse, Joerg %A Winkler, Jana Barbro %A Alfarraj, Saleh %A Albasher, Gadah %A Schnitzler, Joerg-Peter %A Ache, Peter %A Hedrich, Rainer %A Rennenberg, Heinz %D 2021 %I Oxford University Press ({OUP}) %J Tree Physiology %R 10.1093/treephys/tpab027 %T Metabolic responses of date palm (Phoenix dactylifera L.) leaves to drought differ in summer and winter climate

• L{ó}pez-Marqu{é}s, R.L., Norrevang, A.F., Ache, P., Moog, M., Visintainer, D., Wendt, T., {O}sterberg, J.T., Dockter, C., Jorgensen, M.E., Salvador, A.T., Hedrich, R., Gao, C., Jacobsen, S.E., Shabala, S., und Palmgren, M. (2020) „Prospects for the accelerated improvement of the resilient crop quinoa“, J Exp Bot, 71, 5333–5347, verfügbar unter: https://doi.org/10.1093/jxb/eraa285.
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  Crops tolerant to drought and salt stress may be developed by two approaches. First, major crops may be improved by introducing genes from tolerant plants. For example, many major crops have wild relatives that are more tolerant to drought and high salinity than the cultivated crops, and, once deciphered, the underlying resilience mechanisms could be genetically manipulated to produce crops with improved tolerance. Secondly, some minor (orphan) crops cultivated in marginal areas are already drought and salt tolerant. Improving the agronomic performance of these crops may be an effective way to increase crop and food diversity, and an alternative to engineering tolerance in major crops. Quinoa (Chenopodium quinoa Willd.), a nutritious minor crop that tolerates drought and salinity better than most other crops, is an ideal candidate for both of these approaches. Although quinoa has yet to reach its potential as a fully domesticated crop, breeding efforts to improve the plant have been limited. Molecular and genetic techniques combined with traditional breeding are likely to change this picture. Here we analyse protein-coding sequences in the quinoa genome that are orthologous to domestication genes in established crops. Mutating only a limited number of such genes by targeted mutagenesis appears to be a promising route for accelerating the improvement of quinoa and generating a nutritious high-yielding crop that can meet the future demand for food production in a changing climate.

  @article{LopezMarques:2020:J-Exp-Bot:32643753, abstract = {Crops tolerant to drought and salt stress may be developed by two approaches. First, major crops may be improved by introducing genes from tolerant plants. For example, many major crops have wild relatives that are more tolerant to drought and high salinity than the cultivated crops, and, once deciphered, the underlying resilience mechanisms could be genetically manipulated to produce crops with improved tolerance. Secondly, some minor (orphan) crops cultivated in marginal areas are already drought and salt tolerant. Improving the agronomic performance of these crops may be an effective way to increase crop and food diversity, and an alternative to engineering tolerance in major crops. Quinoa (Chenopodium quinoa Willd.), a nutritious minor crop that tolerates drought and salinity better than most other crops, is an ideal candidate for both of these approaches. Although quinoa has yet to reach its potential as a fully domesticated crop, breeding efforts to improve the plant have been limited. Molecular and genetic techniques combined with traditional breeding are likely to change this picture. Here we analyse protein-coding sequences in the quinoa genome that are orthologous to domestication genes in established crops. Mutating only a limited number of such genes by targeted mutagenesis appears to be a promising route for accelerating the improvement of quinoa and generating a nutritious high-yielding crop that can meet the future demand for food production in a changing climate.}, author = {L{\'o}pez-Marqu{\'e}s, R L and Norrevang, A F and Ache, P and Moog, M and Visintainer, D and Wendt, T and {\O}sterberg, J T and Dockter, C and Jorgensen, M E and Salvador, A T and Hedrich, R and Gao, C and Jacobsen, S E and Shabala, S and Palmgren, M}, journal = {J Exp Bot}, keywords = {myown}, month = {06}, pages = {5333-5347}, title = {Prospects for the accelerated improvement of the resilient crop quinoa}, volume = 71, year = 2020 }

  %0 Journal Article %1 LopezMarques:2020:J-Exp-Bot:32643753 %A L{ó}pez-Marqu{é}s, R L %A Norrevang, A F %A Ache, P %A Moog, M %A Visintainer, D %A Wendt, T %A {O}sterberg, J T %A Dockter, C %A Jorgensen, M E %A Salvador, A T %A Hedrich, R %A Gao, C %A Jacobsen, S E %A Shabala, S %A Palmgren, M %D 2020 %J J Exp Bot %P 5333-5347 %R 10.1093/jxb/eraa285 %T Prospects for the accelerated improvement of the resilient crop quinoa %U https://pubmed.ncbi.nlm.nih.gov/32643753/ %V 71 %X Crops tolerant to drought and salt stress may be developed by two approaches. First, major crops may be improved by introducing genes from tolerant plants. For example, many major crops have wild relatives that are more tolerant to drought and high salinity than the cultivated crops, and, once deciphered, the underlying resilience mechanisms could be genetically manipulated to produce crops with improved tolerance. Secondly, some minor (orphan) crops cultivated in marginal areas are already drought and salt tolerant. Improving the agronomic performance of these crops may be an effective way to increase crop and food diversity, and an alternative to engineering tolerance in major crops. Quinoa (Chenopodium quinoa Willd.), a nutritious minor crop that tolerates drought and salinity better than most other crops, is an ideal candidate for both of these approaches. Although quinoa has yet to reach its potential as a fully domesticated crop, breeding efforts to improve the plant have been limited. Molecular and genetic techniques combined with traditional breeding are likely to change this picture. Here we analyse protein-coding sequences in the quinoa genome that are orthologous to domestication genes in established crops. Mutating only a limited number of such genes by targeted mutagenesis appears to be a promising route for accelerating the improvement of quinoa and generating a nutritious high-yielding crop that can meet the future demand for food production in a changing climate.

• Dittrich, M., Mueller, H.M., Bauer, H., Peirats-Llobet, M., Rodriguez, P.L., Geilfus, C.M., Carpentier, S.C., Al Rasheid, K.A.S., Kollist, H., Merilo, E., Herrmann, J., M{ü}ller, T., Ache, P., Hetherington, A.M., und Hedrich, R. (2019) „The role of Arabidopsis ABA receptors from the PYR/PYL/RCAR family in stomatal acclimation and closure signal integration“, Nat Plants, 5(9), 1002–1011, verfügbar unter: https://doi.org/10.1038/s41477-019-0490-0.
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  Stomata are microscopic pores found on the surfaces of leaves that act to control CO2 uptake and water loss. By integrating information derived from endogenous signals with cues from the surrounding environment, the guard cells, which surround the pore, 'set' the stomatal aperture to suit the prevailing conditions. Much research has concentrated on understanding the rapid intracellular changes that result in immediate changes to the stomatal aperture. In this study, we look instead at how stomata acclimate to longer timescale variations in their environment. We show that the closure-inducing signals abscisic acid (ABA), increased CO2, decreased relative air humidity and darkness each access a unique gene network made up of clusters (or modules) of common cellular processes. However, within these networks some gene clusters are shared amongst all four stimuli. All stimuli modulate the expression of members of the PYR/PYL/RCAR family of ABA receptors. However, they are modulated differentially in a stimulus-specific manner. Of the six members of the PYR/PYL/RCAR family expressed in guard cells, PYL2 is sufficient for guard cell ABA-induced responses, whereas in the responses to CO2, PYL4 and PYL5 are essential. Overall, our work shows the importance of ABA as a central regulator and integrator of long-term changes in stomatal behaviour, including sensitivity, elicited by external signals. Understanding this architecture may aid in breeding crops with improved water and nutrient efficiency.

  @article{Dittrich:2019:Nat-Plants:31451795, abstract = {Stomata are microscopic pores found on the surfaces of leaves that act to control CO2 uptake and water loss. By integrating information derived from endogenous signals with cues from the surrounding environment, the guard cells, which surround the pore, 'set' the stomatal aperture to suit the prevailing conditions. Much research has concentrated on understanding the rapid intracellular changes that result in immediate changes to the stomatal aperture. In this study, we look instead at how stomata acclimate to longer timescale variations in their environment. We show that the closure-inducing signals abscisic acid (ABA), increased CO2, decreased relative air humidity and darkness each access a unique gene network made up of clusters (or modules) of common cellular processes. However, within these networks some gene clusters are shared amongst all four stimuli. All stimuli modulate the expression of members of the PYR/PYL/RCAR family of ABA receptors. However, they are modulated differentially in a stimulus-specific manner. Of the six members of the PYR/PYL/RCAR family expressed in guard cells, PYL2 is sufficient for guard cell ABA-induced responses, whereas in the responses to CO2, PYL4 and PYL5 are essential. Overall, our work shows the importance of ABA as a central regulator and integrator of long-term changes in stomatal behaviour, including sensitivity, elicited by external signals. Understanding this architecture may aid in breeding crops with improved water and nutrient efficiency.}, author = {Dittrich, M and Mueller, H M and Bauer, H and Peirats-Llobet, M and Rodriguez, P L and Geilfus, C M and Carpentier, S C and Al Rasheid, K A S and Kollist, H and Merilo, E and Herrmann, J and M{\"u}ller, T and Ache, P and Hetherington, A M and Hedrich, R}, journal = {Nat Plants}, keywords = {imported}, month = {09}, number = 9, pages = {1002-1011}, title = {The role of Arabidopsis ABA receptors from the PYR/PYL/RCAR family in stomatal acclimation and closure signal integration}, volume = 5, year = 2019 }

  %0 Journal Article %1 Dittrich:2019:Nat-Plants:31451795 %A Dittrich, M %A Mueller, H M %A Bauer, H %A Peirats-Llobet, M %A Rodriguez, P L %A Geilfus, C M %A Carpentier, S C %A Al Rasheid, K A S %A Kollist, H %A Merilo, E %A Herrmann, J %A M{ü}ller, T %A Ache, P %A Hetherington, A M %A Hedrich, R %D 2019 %J Nat Plants %N 9 %P 1002-1011 %R 10.1038/s41477-019-0490-0 %T The role of Arabidopsis ABA receptors from the PYR/PYL/RCAR family in stomatal acclimation and closure signal integration %U https://www.ncbi.nlm.nih.gov/pubmed/31451795 %V 5 %X Stomata are microscopic pores found on the surfaces of leaves that act to control CO2 uptake and water loss. By integrating information derived from endogenous signals with cues from the surrounding environment, the guard cells, which surround the pore, 'set' the stomatal aperture to suit the prevailing conditions. Much research has concentrated on understanding the rapid intracellular changes that result in immediate changes to the stomatal aperture. In this study, we look instead at how stomata acclimate to longer timescale variations in their environment. We show that the closure-inducing signals abscisic acid (ABA), increased CO2, decreased relative air humidity and darkness each access a unique gene network made up of clusters (or modules) of common cellular processes. However, within these networks some gene clusters are shared amongst all four stimuli. All stimuli modulate the expression of members of the PYR/PYL/RCAR family of ABA receptors. However, they are modulated differentially in a stimulus-specific manner. Of the six members of the PYR/PYL/RCAR family expressed in guard cells, PYL2 is sufficient for guard cell ABA-induced responses, whereas in the responses to CO2, PYL4 and PYL5 are essential. Overall, our work shows the importance of ABA as a central regulator and integrator of long-term changes in stomatal behaviour, including sensitivity, elicited by external signals. Understanding this architecture may aid in breeding crops with improved water and nutrient efficiency.
• Du, B., Kruse, J., Winkler, J., Alfarray, S., Schnitzler, J., Ache, P., Hedrich, R., und Rennenberg, H. (2019) „Climate and development modulate the metabolome and anti-oxidative system of date palm leaves“, J Exp Bot, 70, 5959–5969, verfügbar unter: https://doi.org/10.1093/jxb/erz361.
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  @article{noauthororeditor, author = {Du, B and Kruse, J and Winkler, JB and Alfarray, S and Schnitzler, JP and Ache, P and Hedrich, R and Rennenberg, H}, journal = {J Exp Bot}, keywords = {imported}, pages = {5959-5969}, title = {Climate and development modulate the metabolome and anti-oxidative system of date palm leaves}, volume = 70, year = 2019 }

  %0 Journal Article %1 noauthororeditor %A Du, B %A Kruse, J %A Winkler, JB %A Alfarray, S %A Schnitzler, JP %A Ache, P %A Hedrich, R %A Rennenberg, H %D 2019 %J J Exp Bot %P 5959-5969 %R 10.1093/jxb/erz361 %T Climate and development modulate the metabolome and anti-oxidative system of date palm leaves %U https://www.ncbi.nlm.nih.gov/pubmed/31375818 %V 70

• Schäfer, N., Maierhofer, T., Herrmann, J., Jorgensen, M.E., Lind, C., von Meyer, K., Lautner, S., Fromm, J., Felder, M., Hetherington, A.M., Ache, P., Geiger, D., und Hedrich, R. (2018) „A Tandem Amino Acid Residue Motif in Guard Cell SLAC1 Anion Channel of Grasses Allows for the Control of Stomatal Aperture by Nitrate“, Curr Biol, 28(9), 1370–1379, verfügbar unter: https://doi.org/10.1016/j.cub.2018.03.027.
  • [ Abstract ]
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  The latest major group of plants to evolve were the grasses. These became important in the mid-Paleogene�about 40 million years ago. During evolution, leaf CO2 uptake and transpirational water loss were optimized by the acquisition of grass-specific stomatal complexes. In contrast to the kidney-shaped�guard cells (GCs) typical of the dicots such as Arabidopsis, in the grasses and agronomically important cereals, the GCs are dumbbell shaped and are associated with morphologically distinct subsidiary cells (SCs). We studied the molecular basis of GC action in the major cereal crop barley. Upon feeding ABA to xylem sap of an intact barley leaf, stomata closed in a nitrate-dependent manner. This process was initiated by activation of GC SLAC-type anion channel currents. HvSLAC1 expressed in Xenopus oocytes gave rise to S-type anion currents that increased several-fold upon stimulation with >3�mM nitrate. We identified a tandem amino acid residue motif that within the SLAC1 channels differs fundamentally between monocots and dicots. When the motif of nitrate-insensitive dicot Arabidopsis SLAC1 was replaced by the monocot signature, AtSLAC1 converted into a grass-type like nitrate-sensitive channel. Our work reveals a fundamental difference between monocot and dicot GCs and prompts questions into the selective pressures during evolution that resulted in fundamental changes in the regulation of SLAC1 function.

  @article{Schafer:2018:Curr-Biol:29706511, abstract = {The latest major group of plants to evolve were the grasses. These became important in the mid-Paleogene�about 40 million years ago. During evolution, leaf CO2 uptake and transpirational water loss were optimized by the acquisition of grass-specific stomatal complexes. In contrast to the kidney-shaped�guard cells (GCs) typical of the dicots such as Arabidopsis, in the grasses and agronomically important cereals, the GCs are dumbbell shaped and are associated with morphologically distinct subsidiary cells (SCs). We studied the molecular basis of GC action in the major cereal crop barley. Upon feeding ABA to xylem sap of an intact barley leaf, stomata closed in a nitrate-dependent manner. This process was initiated by activation of GC SLAC-type anion channel currents. HvSLAC1 expressed in Xenopus oocytes gave rise to S-type anion currents that increased several-fold upon stimulation with >3�mM nitrate. We identified a tandem amino acid residue motif that within the SLAC1 channels differs fundamentally between monocots and dicots. When the motif of nitrate-insensitive dicot Arabidopsis SLAC1 was replaced by the monocot signature, AtSLAC1 converted into a grass-type like nitrate-sensitive channel. Our work reveals a fundamental difference between monocot and dicot GCs and prompts questions into the selective pressures during evolution that resulted in fundamental changes in the regulation of SLAC1 function.}, author = {Schäfer, N and Maierhofer, T and Herrmann, J and Jorgensen, M E and Lind, C and von Meyer, K and Lautner, S and Fromm, J and Felder, M and Hetherington, A M and Ache, P and Geiger, D and Hedrich, R}, journal = {Curr Biol}, keywords = {myown}, month = {05}, number = 9, pages = {1370-1379}, title = {A Tandem Amino Acid Residue Motif in Guard Cell SLAC1 Anion Channel of Grasses Allows for the Control of Stomatal Aperture by Nitrate}, volume = 28, year = 2018 }

  %0 Journal Article %1 Schafer:2018:Curr-Biol:29706511 %A Schäfer, N %A Maierhofer, T %A Herrmann, J %A Jorgensen, M E %A Lind, C %A von Meyer, K %A Lautner, S %A Fromm, J %A Felder, M %A Hetherington, A M %A Ache, P %A Geiger, D %A Hedrich, R %D 2018 %J Curr Biol %N 9 %P 1370-1379 %R 10.1016/j.cub.2018.03.027 %T A Tandem Amino Acid Residue Motif in Guard Cell SLAC1 Anion Channel of Grasses Allows for the Control of Stomatal Aperture by Nitrate %U https://www.ncbi.nlm.nih.gov/pubmed/29706511 %V 28 %X The latest major group of plants to evolve were the grasses. These became important in the mid-Paleogene�about 40 million years ago. During evolution, leaf CO2 uptake and transpirational water loss were optimized by the acquisition of grass-specific stomatal complexes. In contrast to the kidney-shaped�guard cells (GCs) typical of the dicots such as Arabidopsis, in the grasses and agronomically important cereals, the GCs are dumbbell shaped and are associated with morphologically distinct subsidiary cells (SCs). We studied the molecular basis of GC action in the major cereal crop barley. Upon feeding ABA to xylem sap of an intact barley leaf, stomata closed in a nitrate-dependent manner. This process was initiated by activation of GC SLAC-type anion channel currents. HvSLAC1 expressed in Xenopus oocytes gave rise to S-type anion currents that increased several-fold upon stimulation with >3�mM nitrate. We identified a tandem amino acid residue motif that within the SLAC1 channels differs fundamentally between monocots and dicots. When the motif of nitrate-insensitive dicot Arabidopsis SLAC1 was replaced by the monocot signature, AtSLAC1 converted into a grass-type like nitrate-sensitive channel. Our work reveals a fundamental difference between monocot and dicot GCs and prompts questions into the selective pressures during evolution that resulted in fundamental changes in the regulation of SLAC1 function.
• B{ö}hm, J., Messerer, M., M{ü}ller, H.M., Scholz-Starke, J., Gradogna, A., Scherzer, S., Maierhofer, T., Bazihizina, N., Zhang, H., Stigloher, C., Ache, P., Al-Rasheid, K.A.S., Mayer, K.F.X., Shabala, S., Carpaneto, A., Haberer, G., Zhu, J.K., und Hedrich, R. (2018) „Understanding the Molecular Basis of Salt Sequestration in Epidermal Bladder Cells of Chenopodium quinoa“, Curr Biol, 28(19), 3075–3085, verfügbar unter: https://doi.org/10.1016/j.cub.2018.08.004.
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  Soil salinity is destroying arable land and is considered to be one of the major threats to global food security in the 21st century. Therefore, the ability of naturally salt-tolerant halophyte plants to sequester large quantities of salt in external structures, such as epidermal bladder cells (EBCs), is of great interest. Using Chenopodium quinoa, a pseudo-cereal halophyte of great economic potential, we have shown previously that, upon removal of salt bladders, quinoa becomes salt sensitive. In this work, we analyzed the molecular mechanism underlying the unique salt dumping capabilities of bladder cells in quinoa. The transporters differentially expressed in the EBC transcriptome and functional electrophysiological testing of key EBC transporters in Xenopus oocytes revealed that loading of Na+ and Cl- into EBCs is mediated by a set of tailored plasma and vacuole membrane-based sodium-selective channel and chloride-permeable transporter.

  @article{Bohm:2018:Curr-Biol:30245105, abstract = {Soil salinity is destroying arable land and is considered to be one of the major threats to global food security in the 21st century. Therefore, the ability of naturally salt-tolerant halophyte plants to sequester large quantities of salt in external structures, such as epidermal bladder cells (EBCs), is of great interest. Using Chenopodium quinoa, a pseudo-cereal halophyte of great economic potential, we have shown previously that, upon removal of salt bladders, quinoa becomes salt sensitive. In this work, we analyzed the molecular mechanism underlying the unique salt dumping capabilities of bladder cells in quinoa. The transporters differentially expressed in the EBC transcriptome and functional electrophysiological testing of key EBC transporters in Xenopus oocytes revealed that loading of Na+ and Cl- into EBCs is mediated by a set of tailored plasma and vacuole membrane-based sodium-selective channel and chloride-permeable transporter.}, author = {B{\"o}hm, J and Messerer, M and M{\"u}ller, H M and Scholz-Starke, J and Gradogna, A and Scherzer, S and Maierhofer, T and Bazihizina, N and Zhang, H and Stigloher, C and Ache, P and Al-Rasheid, K A S and Mayer, K F X and Shabala, S and Carpaneto, A and Haberer, G and Zhu, J K and Hedrich, R}, journal = {Curr Biol}, keywords = {imported}, month = 10, number = 19, pages = {3075-3085}, title = {Understanding the Molecular Basis of Salt Sequestration in Epidermal Bladder Cells of Chenopodium quinoa}, volume = 28, year = 2018 }

  %0 Journal Article %1 Bohm:2018:Curr-Biol:30245105 %A B{ö}hm, J %A Messerer, M %A M{ü}ller, H M %A Scholz-Starke, J %A Gradogna, A %A Scherzer, S %A Maierhofer, T %A Bazihizina, N %A Zhang, H %A Stigloher, C %A Ache, P %A Al-Rasheid, K A S %A Mayer, K F X %A Shabala, S %A Carpaneto, A %A Haberer, G %A Zhu, J K %A Hedrich, R %D 2018 %J Curr Biol %N 19 %P 3075-3085 %R 10.1016/j.cub.2018.08.004 %T Understanding the Molecular Basis of Salt Sequestration in Epidermal Bladder Cells of Chenopodium quinoa %U https://www.ncbi.nlm.nih.gov/pubmed/30245105 %V 28 %X Soil salinity is destroying arable land and is considered to be one of the major threats to global food security in the 21st century. Therefore, the ability of naturally salt-tolerant halophyte plants to sequester large quantities of salt in external structures, such as epidermal bladder cells (EBCs), is of great interest. Using Chenopodium quinoa, a pseudo-cereal halophyte of great economic potential, we have shown previously that, upon removal of salt bladders, quinoa becomes salt sensitive. In this work, we analyzed the molecular mechanism underlying the unique salt dumping capabilities of bladder cells in quinoa. The transporters differentially expressed in the EBC transcriptome and functional electrophysiological testing of key EBC transporters in Xenopus oocytes revealed that loading of Na+ and Cl- into EBCs is mediated by a set of tailored plasma and vacuole membrane-based sodium-selective channel and chloride-permeable transporter.
• Du, B., Kreuzwieser, J., Winkler, J.B., Ghirardo, A., Schnitzler, J.P., Ache, P., Alfarraj, S., Hedrich, R., White, P., und Rennenberg, H. (2018) „Physiological responses of date palm (Phoenix dactylifera) seedlings to acute ozone exposure at high temperature“, Environ Pollut, 242(Pt A), 905–913, verfügbar unter: https://doi.org/10.1016/j.envpol.2018.07.059.
  • [ Abstract ]
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  Vegetation in the Arabian Peninsula is facing high and steadily rising tropospheric ozone pollution. However, little is known about the impacts of elevated ozone on date palms, one of the most important indigenous economic species. To elucidate the physiological responses of date palm to peak levels of acute ozone exposure, seedlings were fumigated with 200 ppb ozone for 8 h. Net CO2 assimilation rate, stomatal conduction, total carbon, its isotope signature and total sugar contents in leaves and roots were not significantly affected by the treatment and visible symptoms of foliar damage were not induced. Ozone exposure did not affect hydrogen peroxide and thiol contents but diminished the activities of glutathione reductase and dehydroascorbate reductase, stimulated the oxidation of ascorbate, and resulted in elevated total ascorbate contents. Total nitrogen, soluble protein and lignin contents remained unchanged upon ozone exposure, but the abundance of low molecular weight nitrogen (LMWN) compounds such as amino acids and nitrate as well as other anions were strongly diminished in leaves and roots. Other nitrogen pools did not benefit from the decline of LMWN, indicating reduced uptake and/or enhanced release of these compounds into the soil as a systemic response to aboveground ozone exposure. Several phenolic compounds, concurrent with fatty acids and stearyl alcohol, accumulated in leaves, but declined in roots, whereas total phenol contents significantly increased in the roots. Together these results indicate that local and systemic changes in both, primary and secondary metabolism contribute to the high tolerance of date palms to short-term acute ozone exposure.

  @article{Du:2018:Environ-Pollut:30041163, abstract = {Vegetation in the Arabian Peninsula is facing high and steadily rising tropospheric ozone pollution. However, little is known about the impacts of elevated ozone on date palms, one of the most important indigenous economic species. To elucidate the physiological responses of date palm to peak levels of acute ozone exposure, seedlings were fumigated with 200 ppb ozone for 8 h. Net CO2 assimilation rate, stomatal conduction, total carbon, its isotope signature and total sugar contents in leaves and roots were not significantly affected by the treatment and visible symptoms of foliar damage were not induced. Ozone exposure did not affect hydrogen peroxide and thiol contents but diminished the activities of glutathione reductase and dehydroascorbate reductase, stimulated the oxidation of ascorbate, and resulted in elevated total ascorbate contents. Total nitrogen, soluble protein and lignin contents remained unchanged upon ozone exposure, but the abundance of low molecular weight nitrogen (LMWN) compounds such as amino acids and nitrate as well as other anions were strongly diminished in leaves and roots. Other nitrogen pools did not benefit from the decline of LMWN, indicating reduced uptake and/or enhanced release of these compounds into the soil as a systemic response to aboveground ozone exposure. Several phenolic compounds, concurrent with fatty acids and stearyl alcohol, accumulated in leaves, but declined in roots, whereas total phenol contents significantly increased in the roots. Together these results indicate that local and systemic changes in both, primary and secondary metabolism contribute to the high tolerance of date palms to short-term acute ozone exposure.}, author = {Du, B and Kreuzwieser, J and Winkler, J B and Ghirardo, A and Schnitzler, J P and Ache, P and Alfarraj, S and Hedrich, R and White, P and Rennenberg, H}, journal = {Environ Pollut}, keywords = {imported}, month = 11, number = {Pt A}, pages = {905-913}, title = {Physiological responses of date palm (Phoenix dactylifera) seedlings to acute ozone exposure at high temperature}, volume = 242, year = 2018 }

  %0 Journal Article %1 Du:2018:Environ-Pollut:30041163 %A Du, B %A Kreuzwieser, J %A Winkler, J B %A Ghirardo, A %A Schnitzler, J P %A Ache, P %A Alfarraj, S %A Hedrich, R %A White, P %A Rennenberg, H %D 2018 %J Environ Pollut %N Pt A %P 905-913 %R 10.1016/j.envpol.2018.07.059 %T Physiological responses of date palm (Phoenix dactylifera) seedlings to acute ozone exposure at high temperature %U https://www.ncbi.nlm.nih.gov/pubmed/30041163 %V 242 %X Vegetation in the Arabian Peninsula is facing high and steadily rising tropospheric ozone pollution. However, little is known about the impacts of elevated ozone on date palms, one of the most important indigenous economic species. To elucidate the physiological responses of date palm to peak levels of acute ozone exposure, seedlings were fumigated with 200 ppb ozone for 8 h. Net CO2 assimilation rate, stomatal conduction, total carbon, its isotope signature and total sugar contents in leaves and roots were not significantly affected by the treatment and visible symptoms of foliar damage were not induced. Ozone exposure did not affect hydrogen peroxide and thiol contents but diminished the activities of glutathione reductase and dehydroascorbate reductase, stimulated the oxidation of ascorbate, and resulted in elevated total ascorbate contents. Total nitrogen, soluble protein and lignin contents remained unchanged upon ozone exposure, but the abundance of low molecular weight nitrogen (LMWN) compounds such as amino acids and nitrate as well as other anions were strongly diminished in leaves and roots. Other nitrogen pools did not benefit from the decline of LMWN, indicating reduced uptake and/or enhanced release of these compounds into the soil as a systemic response to aboveground ozone exposure. Several phenolic compounds, concurrent with fatty acids and stearyl alcohol, accumulated in leaves, but declined in roots, whereas total phenol contents significantly increased in the roots. Together these results indicate that local and systemic changes in both, primary and secondary metabolism contribute to the high tolerance of date palms to short-term acute ozone exposure.

• Zou, C., Chen, A., Xiao, L., Muller, H.M., Ache, P., Haberer, G., Zhang, M., Jia, W., Deng, P., Huang, R., Lang, D., Li, F., Zhan, D., Wu, X., Zhang, H., Bohm, J., Liu, R., Shabala, S., Hedrich, R., Zhu, J.K., und Zhang, H. (2017) „A high-quality genome assembly of quinoa provides insights into the molecular basis of salt bladder-based salinity tolerance and the exceptional nutritional value“, Cell Res, 27, 1327–1340, verfügbar unter: https://doi.org/10.1038/cr.2017.124.
  • [ Abstract ]
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  Chenopodium quinoa is a halophytic pseudocereal crop that is being cultivated in an ever-growing number of countries. Because quinoa is highly resistant to multiple abiotic stresses and its seed has a better nutritional value than any other major cereals, it is regarded as a future crop to ensure global food security. We generated a high-quality genome draft using an inbred line of the quinoa cultivar Real. The quinoa genome experienced one recent genome duplication about 4.3 million years ago, likely reflecting the genome fusion of two Chenopodium parents, in addition to the γ paleohexaploidization reported for most eudicots. The genome is highly repetitive (64.5% repeat content) and contains 54 438 protein-coding genes and 192 microRNA genes, with more than 99.3% having orthologous genes from glycophylic species. Stress tolerance in quinoa is associated with the expansion of genes involved in ion and nutrient transport, ABA homeostasis and signaling, and enhanced basal-level ABA responses. Epidermal salt bladder cells exhibit similar characteristics as trichomes, with a significantly higher expression of genes related to energy import and ABA biosynthesis compared with the leaf lamina. The quinoa genome sequence provides insights into its exceptional nutritional value and the evolution of halophytes, enabling the identification of genes involved in salinity tolerance, and providing the basis for molecular breeding in quinoa.Cell Research advance online publication 10 October 2017; doi:10.1038/cr.2017.124.

  @article{Zou:2017:Cell-Res:28994416, abstract = {Chenopodium quinoa is a halophytic pseudocereal crop that is being cultivated in an ever-growing number of countries. Because quinoa is highly resistant to multiple abiotic stresses and its seed has a better nutritional value than any other major cereals, it is regarded as a future crop to ensure global food security. We generated a high-quality genome draft using an inbred line of the quinoa cultivar Real. The quinoa genome experienced one recent genome duplication about 4.3 million years ago, likely reflecting the genome fusion of two Chenopodium parents, in addition to the γ paleohexaploidization reported for most eudicots. The genome is highly repetitive (64.5% repeat content) and contains 54 438 protein-coding genes and 192 microRNA genes, with more than 99.3% having orthologous genes from glycophylic species. Stress tolerance in quinoa is associated with the expansion of genes involved in ion and nutrient transport, ABA homeostasis and signaling, and enhanced basal-level ABA responses. Epidermal salt bladder cells exhibit similar characteristics as trichomes, with a significantly higher expression of genes related to energy import and ABA biosynthesis compared with the leaf lamina. The quinoa genome sequence provides insights into its exceptional nutritional value and the evolution of halophytes, enabling the identification of genes involved in salinity tolerance, and providing the basis for molecular breeding in quinoa.Cell Research advance online publication 10 October 2017; doi:10.1038/cr.2017.124.}, author = {Zou, C and Chen, A and Xiao, L and Muller, H M and Ache, P and Haberer, G and Zhang, M and Jia, W and Deng, P and Huang, R and Lang, D and Li, F and Zhan, D and Wu, X and Zhang, H and Bohm, J and Liu, R and Shabala, S and Hedrich, R and Zhu, J K and Zhang, H}, journal = {Cell Res}, keywords = {myown}, month = 10, pages = {1327-1340}, title = {A high-quality genome assembly of quinoa provides insights into the molecular basis of salt bladder-based salinity tolerance and the exceptional nutritional value}, volume = 27, year = 2017 }

  %0 Journal Article %1 Zou:2017:Cell-Res:28994416 %A Zou, C %A Chen, A %A Xiao, L %A Muller, H M %A Ache, P %A Haberer, G %A Zhang, M %A Jia, W %A Deng, P %A Huang, R %A Lang, D %A Li, F %A Zhan, D %A Wu, X %A Zhang, H %A Bohm, J %A Liu, R %A Shabala, S %A Hedrich, R %A Zhu, J K %A Zhang, H %D 2017 %J Cell Res %P 1327-1340 %R 10.1038/cr.2017.124 %T A high-quality genome assembly of quinoa provides insights into the molecular basis of salt bladder-based salinity tolerance and the exceptional nutritional value %U https://www.ncbi.nlm.nih.gov/pubmed/28994416 %V 27 %X Chenopodium quinoa is a halophytic pseudocereal crop that is being cultivated in an ever-growing number of countries. Because quinoa is highly resistant to multiple abiotic stresses and its seed has a better nutritional value than any other major cereals, it is regarded as a future crop to ensure global food security. We generated a high-quality genome draft using an inbred line of the quinoa cultivar Real. The quinoa genome experienced one recent genome duplication about 4.3 million years ago, likely reflecting the genome fusion of two Chenopodium parents, in addition to the γ paleohexaploidization reported for most eudicots. The genome is highly repetitive (64.5% repeat content) and contains 54 438 protein-coding genes and 192 microRNA genes, with more than 99.3% having orthologous genes from glycophylic species. Stress tolerance in quinoa is associated with the expansion of genes involved in ion and nutrient transport, ABA homeostasis and signaling, and enhanced basal-level ABA responses. Epidermal salt bladder cells exhibit similar characteristics as trichomes, with a significantly higher expression of genes related to energy import and ABA biosynthesis compared with the leaf lamina. The quinoa genome sequence provides insights into its exceptional nutritional value and the evolution of halophytes, enabling the identification of genes involved in salinity tolerance, and providing the basis for molecular breeding in quinoa.Cell Research advance online publication 10 October 2017; doi:10.1038/cr.2017.124.
• M{ü}ller, H.M., Sch{ä}fer, N., Bauer, H., Geiger, D., Lautner, S., Fromm, J., Riederer, M., Bueno, A., Nussbaumer, T., Mayer, K., Alquraishi, S.A., Alfarhan, A.H., Neher, E., Al-Rasheid, K.A.S., Ache, P., und Hedrich, R. (2017) „The desert plant Phoenix dactylifera closes stomata via nitrate-regulated SLAC1 anion channel“, New Phytol, 216, 150–162, verfügbar unter: https://doi.org/10.1111/nph.14672.
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  • [ DOI ]
  Date palm Phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. How are palms under such harsh environmental conditions able to limit transpirational water loss? Here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from P.�dactylifera. To understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast abscisic acid (ABA) signalling and reconstituted this ABA signalosome in Xenopus oocytes. The PhoenixSLAC1-type anion channel is regulated by ABA kinase PdOST1. Energy-dispersive X-ray analysis (EDXA) demonstrated that date palm guard cells release chloride during stomatal closure. However, in Cl(-) medium, PdOST1 did not activate the desert plant anion channel PdSLAC1 per�se. Only when nitrate was present at the extracellular face of the anion channel did the OST1-gated PdSLAC1 open, thus enabling chloride release. In the presence of nitrate, ABA enhanced and accelerated stomatal closure. Our findings indicate that, in date palm, the guard cell osmotic motor driving stomatal closure uses nitrate as the signal to open the major anion channel SLAC1. This initiates guard cell depolarization and the release of anions together with potassium.

  @article{Muller:2017:New-Phytol:28670699, abstract = {Date palm Phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. How are palms under such harsh environmental conditions able to limit transpirational water loss? Here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from P.�dactylifera. To understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast abscisic acid (ABA) signalling and reconstituted this ABA signalosome in Xenopus oocytes. The PhoenixSLAC1-type anion channel is regulated by ABA kinase PdOST1. Energy-dispersive X-ray analysis (EDXA) demonstrated that date palm guard cells release chloride during stomatal closure. However, in Cl(-) medium, PdOST1 did not activate the desert plant anion channel PdSLAC1 per�se. Only when nitrate was present at the extracellular face of the anion channel did the OST1-gated PdSLAC1 open, thus enabling chloride release. In the presence of nitrate, ABA enhanced and accelerated stomatal closure. Our findings indicate that, in date palm, the guard cell osmotic motor driving stomatal closure uses nitrate as the signal to open the major anion channel SLAC1. This initiates guard cell depolarization and the release of anions together with potassium.}, author = {M{\"u}ller, H M and Sch{\"a}fer, N and Bauer, H and Geiger, D and Lautner, S and Fromm, J and Riederer, M and Bueno, A and Nussbaumer, T and Mayer, K and Alquraishi, S A and Alfarhan, A H and Neher, E and Al-Rasheid, K A S and Ache, P and Hedrich, R}, journal = {New Phytol}, keywords = {myown}, month = {07}, pages = {150-162}, title = {The desert plant Phoenix dactylifera closes stomata via nitrate-regulated SLAC1 anion channel}, volume = 216, year = 2017 }

  %0 Journal Article %1 Muller:2017:New-Phytol:28670699 %A M{ü}ller, H M %A Sch{ä}fer, N %A Bauer, H %A Geiger, D %A Lautner, S %A Fromm, J %A Riederer, M %A Bueno, A %A Nussbaumer, T %A Mayer, K %A Alquraishi, S A %A Alfarhan, A H %A Neher, E %A Al-Rasheid, K A S %A Ache, P %A Hedrich, R %D 2017 %J New Phytol %P 150-162 %R 10.1111/nph.14672 %T The desert plant Phoenix dactylifera closes stomata via nitrate-regulated SLAC1 anion channel %U https://www.ncbi.nlm.nih.gov/pubmed/28670699 %V 216 %X Date palm Phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. How are palms under such harsh environmental conditions able to limit transpirational water loss? Here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from P.�dactylifera. To understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast abscisic acid (ABA) signalling and reconstituted this ABA signalosome in Xenopus oocytes. The PhoenixSLAC1-type anion channel is regulated by ABA kinase PdOST1. Energy-dispersive X-ray analysis (EDXA) demonstrated that date palm guard cells release chloride during stomatal closure. However, in Cl(-) medium, PdOST1 did not activate the desert plant anion channel PdSLAC1 per�se. Only when nitrate was present at the extracellular face of the anion channel did the OST1-gated PdSLAC1 open, thus enabling chloride release. In the presence of nitrate, ABA enhanced and accelerated stomatal closure. Our findings indicate that, in date palm, the guard cell osmotic motor driving stomatal closure uses nitrate as the signal to open the major anion channel SLAC1. This initiates guard cell depolarization and the release of anions together with potassium.
• Malcheska, F., Ahmad, A., Batool, S., M{ü}ller, H.M., Ludwig-M{ü}ller, J., Kreuzwieser, J., Randewig, D., H{ä}nsch, R., Mendel, R.R., Hell, R., Wirtz, M., Geiger, D., Ache, P., Hedrich, R., Herschbach, C., und Rennenberg, H. (2017) „Drought enhanced xylem sap sulfate closes stomata by affecting ALMT12 and guard cell ABA synthesis“, Plant Physiol, 174, 798–814, verfügbar unter: https://doi.org/10.1104/pp.16.01784.
  • [ Abstract ]
  • [ BibTeX ]
  • [ EndNote ]
  • [ URL ]
  • [ DOI ]
  Water limitation of plants causes stomatal closure to prevent water loss by transpiration. For this purpose, progressing soil water deficit is communicated from roots to shoots. Abscisic acid (ABA) is the key signal in stress-induced stomatal closure, however, ABA as early xylem-delivered signal is still a matter of debate. In the present study, poplar plants were exposed to water stress to investigate xylem sap sulfate and ABA, stomatal conductance and sulfate transporter (SULTR) expression. In addition, stomatal behavior and expression of ABA receptors, drought responsive genes, transcription factors and NCED3 were studied after feeding sulfate and ABA to detached poplar leaves and epidermal peels of Arabidopsis. The results show that increased xylem sap sulfate is achieved upon drought by reduced xylem unloading by PtaSULTR3;3a and PtaSULTR1;1, and by enhanced loading from parenchyma cells into the xylem via PtaALMT3b. Sulfate application caused stomatal closure in excised leaves and peeled epidermis. In the loss of sulfate-channel function mutant, Atalmt12, sulfate-triggered stomatal closure was impaired. The QUAC1/ALMT12 anion channel heterologous expressed in oocytes was gated open by extracellular sulfate. Sulfate up-regulated the expression of NCED3, a key step of ABA synthesis, in guard cells. In conclusion, xylem-derived sulfate seems an early chemical signal of drought inducing stomatal closure via QUAC1/ALMT12 and/or guard cell ABA synthesis.

  @article{Malcheska:2017:Plant-Physiol:28446637, abstract = {Water limitation of plants causes stomatal closure to prevent water loss by transpiration. For this purpose, progressing soil water deficit is communicated from roots to shoots. Abscisic acid (ABA) is the key signal in stress-induced stomatal closure, however, ABA as early xylem-delivered signal is still a matter of debate. In the present study, poplar plants were exposed to water stress to investigate xylem sap sulfate and ABA, stomatal conductance and sulfate transporter (SULTR) expression. In addition, stomatal behavior and expression of ABA receptors, drought responsive genes, transcription factors and NCED3 were studied after feeding sulfate and ABA to detached poplar leaves and epidermal peels of Arabidopsis. The results show that increased xylem sap sulfate is achieved upon drought by reduced xylem unloading by PtaSULTR3;3a and PtaSULTR1;1, and by enhanced loading from parenchyma cells into the xylem via PtaALMT3b. Sulfate application caused stomatal closure in excised leaves and peeled epidermis. In the loss of sulfate-channel function mutant, Atalmt12, sulfate-triggered stomatal closure was impaired. The QUAC1/ALMT12 anion channel heterologous expressed in oocytes was gated open by extracellular sulfate. Sulfate up-regulated the expression of NCED3, a key step of ABA synthesis, in guard cells. In conclusion, xylem-derived sulfate seems an early chemical signal of drought inducing stomatal closure via QUAC1/ALMT12 and/or guard cell ABA synthesis.}, author = {Malcheska, F and Ahmad, A and Batool, S and M{\"u}ller, H M and Ludwig-M{\"u}ller, J and Kreuzwieser, J and Randewig, D and H{\"a}nsch, R and Mendel, R R and Hell, R and Wirtz, M and Geiger, D and Ache, P and Hedrich, R and Herschbach, C and Rennenberg, H}, journal = {Plant Physiol}, keywords = {myown}, month = {04}, pages = {798-814}, title = {Drought enhanced xylem sap sulfate closes stomata by affecting ALMT12 and guard cell ABA synthesis}, volume = 174, year = 2017 }

  %0 Journal Article %1 Malcheska:2017:Plant-Physiol:28446637 %A Malcheska, F %A Ahmad, A %A Batool, S %A M{ü}ller, H M %A Ludwig-M{ü}ller, J %A Kreuzwieser, J %A Randewig, D %A H{ä}nsch, R %A Mendel, R R %A Hell, R %A Wirtz, M %A Geiger, D %A Ache, P %A Hedrich, R %A Herschbach, C %A Rennenberg, H %D 2017 %J Plant Physiol %P 798-814 %R 10.1104/pp.16.01784 %T Drought enhanced xylem sap sulfate closes stomata by affecting ALMT12 and guard cell ABA synthesis %U https://www.ncbi.nlm.nih.gov/pubmed/28446637 %V 174 %X Water limitation of plants causes stomatal closure to prevent water loss by transpiration. For this purpose, progressing soil water deficit is communicated from roots to shoots. Abscisic acid (ABA) is the key signal in stress-induced stomatal closure, however, ABA as early xylem-delivered signal is still a matter of debate. In the present study, poplar plants were exposed to water stress to investigate xylem sap sulfate and ABA, stomatal conductance and sulfate transporter (SULTR) expression. In addition, stomatal behavior and expression of ABA receptors, drought responsive genes, transcription factors and NCED3 were studied after feeding sulfate and ABA to detached poplar leaves and epidermal peels of Arabidopsis. The results show that increased xylem sap sulfate is achieved upon drought by reduced xylem unloading by PtaSULTR3;3a and PtaSULTR1;1, and by enhanced loading from parenchyma cells into the xylem via PtaALMT3b. Sulfate application caused stomatal closure in excised leaves and peeled epidermis. In the loss of sulfate-channel function mutant, Atalmt12, sulfate-triggered stomatal closure was impaired. The QUAC1/ALMT12 anion channel heterologous expressed in oocytes was gated open by extracellular sulfate. Sulfate up-regulated the expression of NCED3, a key step of ABA synthesis, in guard cells. In conclusion, xylem-derived sulfate seems an early chemical signal of drought inducing stomatal closure via QUAC1/ALMT12 and/or guard cell ABA synthesis.
• Safronov, O., Kreuzwieser, J., Haberer, G., Alyousif, M.S., Schulze, W., Al-Harbi, N., Arab, L., Ache, P., Stempfl, T., Kruse, J., Mayer, K.X., Hedrich, R., Rennenberg, H., Saloj{ä}rvi, J., und Kangasj{ä}rvi, J. (2017) „Detecting early signs of heat and drought stress in Phoenix dactylifera (date palm)“, PLoS One, 12, e0177883, verfügbar unter: https://doi.org/10.1371/journal.pone.0177883.
  • [ Abstract ]
  • [ BibTeX ]
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  Plants adapt to the environment by either long-term genome evolution or by acclimatization processes where the cellular processes and metabolism of the plant are adjusted within the existing potential in the genome. Here we studied the adaptation strategies in date palm, Phoenix dactylifera, under mild heat, drought and combined heat and drought by transcriptomic and metabolomic profiling. In transcriptomics data, combined heat and drought resembled heat response, whereas in metabolomics data it was more similar to drought. In both conditions, soluble carbohydrates, such as fucose, and glucose derivatives, were increased, suggesting a switch to carbohydrate metabolism and cell wall biogenesis. This result is consistent with the evidence from transcriptomics and cis-motif analysis. In addition, transcriptomics data showed transcriptional activation of genes related to reactive oxygen species in all three conditions (drought, heat, and combined heat and drought), suggesting increased activity of enzymatic antioxidant systems in cytosol, chloroplast and peroxisome. Finally, the genes that were differentially expressed in heat and combined heat and drought stresses were significantly enriched for circadian and diurnal rhythm motifs, suggesting new stress avoidance strategies.

  @article{Safronov:2017:PLoS-One:28570677, abstract = {Plants adapt to the environment by either long-term genome evolution or by acclimatization processes where the cellular processes and metabolism of the plant are adjusted within the existing potential in the genome. Here we studied the adaptation strategies in date palm, Phoenix dactylifera, under mild heat, drought and combined heat and drought by transcriptomic and metabolomic profiling. In transcriptomics data, combined heat and drought resembled heat response, whereas in metabolomics data it was more similar to drought. In both conditions, soluble carbohydrates, such as fucose, and glucose derivatives, were increased, suggesting a switch to carbohydrate metabolism and cell wall biogenesis. This result is consistent with the evidence from transcriptomics and cis-motif analysis. In addition, transcriptomics data showed transcriptional activation of genes related to reactive oxygen species in all three conditions (drought, heat, and combined heat and drought), suggesting increased activity of enzymatic antioxidant systems in cytosol, chloroplast and peroxisome. Finally, the genes that were differentially expressed in heat and combined heat and drought stresses were significantly enriched for circadian and diurnal rhythm motifs, suggesting new stress avoidance strategies.}, author = {Safronov, O and Kreuzwieser, J and Haberer, G and Alyousif, M S and Schulze, W and Al-Harbi, N and Arab, L and Ache, P and Stempfl, T and Kruse, J and Mayer, K X and Hedrich, R and Rennenberg, H and Saloj{\"a}rvi, J and Kangasj{\"a}rvi, J}, journal = {PLoS One}, keywords = {myown}, pages = {e0177883}, title = {Detecting early signs of heat and drought stress in Phoenix dactylifera (date palm)}, volume = 12, year = 2017 }

  %0 Journal Article %1 Safronov:2017:PLoS-One:28570677 %A Safronov, O %A Kreuzwieser, J %A Haberer, G %A Alyousif, M S %A Schulze, W %A Al-Harbi, N %A Arab, L %A Ache, P %A Stempfl, T %A Kruse, J %A Mayer, K X %A Hedrich, R %A Rennenberg, H %A Saloj{ä}rvi, J %A Kangasj{ä}rvi, J %D 2017 %J PLoS One %P e0177883 %R 10.1371/journal.pone.0177883 %T Detecting early signs of heat and drought stress in Phoenix dactylifera (date palm) %U https://www.ncbi.nlm.nih.gov/pubmed/28570677 %V 12 %X Plants adapt to the environment by either long-term genome evolution or by acclimatization processes where the cellular processes and metabolism of the plant are adjusted within the existing potential in the genome. Here we studied the adaptation strategies in date palm, Phoenix dactylifera, under mild heat, drought and combined heat and drought by transcriptomic and metabolomic profiling. In transcriptomics data, combined heat and drought resembled heat response, whereas in metabolomics data it was more similar to drought. In both conditions, soluble carbohydrates, such as fucose, and glucose derivatives, were increased, suggesting a switch to carbohydrate metabolism and cell wall biogenesis. This result is consistent with the evidence from transcriptomics and cis-motif analysis. In addition, transcriptomics data showed transcriptional activation of genes related to reactive oxygen species in all three conditions (drought, heat, and combined heat and drought), suggesting increased activity of enzymatic antioxidant systems in cytosol, chloroplast and peroxisome. Finally, the genes that were differentially expressed in heat and combined heat and drought stresses were significantly enriched for circadian and diurnal rhythm motifs, suggesting new stress avoidance strategies.

• Jaborsky, M., Maierhofer, T., Olbrich, A., Escalante-Perez, M., Muller, H.M., Simon, J., Krol, E., Cuin, T.A., Fromm, J., Ache, P., Geiger, D., und Hedrich, R. (2016) „SLAH3-type anion channel expressed in poplar secretory epithelia operates in calcium kinase CPK-autonomous manner“, New Phytol, 210, 922–33, verfügbar unter: https://doi.org/10.1111/nph.13841.
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  @article{RN25, author = {Jaborsky, M. and Maierhofer, T. and Olbrich, A. and Escalante-Perez, M. and Muller, H. M. and Simon, J. and Krol, E. and Cuin, T. A. and Fromm, J. and Ache, P. and Geiger, D. and Hedrich, R.}, journal = {New Phytol}, keywords = {myown}, pages = {922-33}, title = {SLAH3-type anion channel expressed in poplar secretory epithelia operates in calcium kinase CPK-autonomous manner}, type = {Journal Article}, volume = 210, year = 2016 }

  %0 Journal Article %1 RN25 %A Jaborsky, M. %A Maierhofer, T. %A Olbrich, A. %A Escalante-Perez, M. %A Muller, H. M. %A Simon, J. %A Krol, E. %A Cuin, T. A. %A Fromm, J. %A Ache, P. %A Geiger, D. %A Hedrich, R. %D 2016 %J New Phytol %P 922-33 %R 10.1111/nph.13841 %T SLAH3-type anion channel expressed in poplar secretory epithelia operates in calcium kinase CPK-autonomous manner %U http://www.ncbi.nlm.nih.gov/pubmed/26831448 %V 210
• Arab, L., Kreuzwieser, J., Kruse, J., Zimmer, I., Ache, P., Alfarraj, S., Al-Rasheid, K.A.S., Schnitzler, J.P., Hedrich, R., und Rennenberg, H. (2016) „Acclimation to heat and drought-Lessons to learn from the date palm (Phoenix dactylifera)“, Environ Exp Bot, 125, 20–30, verfügbar unter: https://doi.org/10.1016/j.envexpbot.2016.01.003.
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  @article{RN5, author = {Arab, L. and Kreuzwieser, J. and Kruse, J. and Zimmer, I. and Ache, P. and Alfarraj, S. and Al-Rasheid, K. A. S. and Schnitzler, J. P. and Hedrich, R. and Rennenberg, H.}, journal = {Environ Exp Bot}, keywords = {myown}, pages = {20-30}, title = {Acclimation to heat and drought-Lessons to learn from the date palm (Phoenix dactylifera)}, type = {Journal Article}, volume = 125, year = 2016 }

  %0 Journal Article %1 RN5 %A Arab, L. %A Kreuzwieser, J. %A Kruse, J. %A Zimmer, I. %A Ache, P. %A Alfarraj, S. %A Al-Rasheid, K. A. S. %A Schnitzler, J. P. %A Hedrich, R. %A Rennenberg, H. %D 2016 %J Environ Exp Bot %P 20-30 %R 10.1016/j.envexpbot.2016.01.003 %T Acclimation to heat and drought-Lessons to learn from the date palm (Phoenix dactylifera) %V 125

• Prasch, C.M., Ott, K.V., Bauer, H., Ache, P., Hedrich, R., und Sonnewald, U. (2015) „ss-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells“, J Exp Bot, 66, 6059–67, verfügbar unter: https://doi.org/10.1093/jxb/erv323.
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  @article{RN33, author = {Prasch, C. M. and Ott, K. V. and Bauer, H. and Ache, P. and Hedrich, R. and Sonnewald, U.}, journal = {J Exp Bot}, keywords = {myown}, pages = {6059-67}, title = {ss-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells}, type = {Journal Article}, volume = 66, year = 2015 }

  %0 Journal Article %1 RN33 %A Prasch, C. M. %A Ott, K. V. %A Bauer, H. %A Ache, P. %A Hedrich, R. %A Sonnewald, U. %D 2015 %J J Exp Bot %P 6059-67 %R 10.1093/jxb/erv323 %T ss-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells %U http://www.ncbi.nlm.nih.gov/pubmed/26139825 %V 66

• Maierhofer, T., Lind, C., Huttl, S., Scherzer, S., Papenfuss, M., Simon, J., Al-Rasheid, K.A.S., Ache, P., Rennenberg, H., Hedrich, R., Muller, T.D., und Geiger, D. (2014) „A single-pore residue renders the Arabidopsis root anion channel SLAH2 highly nitrate selective“, Plant Cell, 26, 2554–2567, verfügbar unter: https://doi.org/10.1105/tpc.114.125849.
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  @article{RN30, author = {Maierhofer, T. and Lind, C. and Huttl, S. and Scherzer, S. and Papenfuss, M. and Simon, J. and Al-Rasheid, K. A. S. and Ache, P. and Rennenberg, H. and Hedrich, R. and Muller, T. D. and Geiger, D.}, journal = {Plant Cell}, keywords = {myown}, pages = {2554-2567}, title = {A single-pore residue renders the Arabidopsis root anion channel SLAH2 highly nitrate selective}, type = {Journal Article}, volume = 26, year = 2014 }

  %0 Journal Article %1 RN30 %A Maierhofer, T. %A Lind, C. %A Huttl, S. %A Scherzer, S. %A Papenfuss, M. %A Simon, J. %A Al-Rasheid, K. A. S. %A Ache, P. %A Rennenberg, H. %A Hedrich, R. %A Muller, T. D. %A Geiger, D. %D 2014 %J Plant Cell %P 2554-2567 %R 10.1105/tpc.114.125849 %T A single-pore residue renders the Arabidopsis root anion channel SLAH2 highly nitrate selective %U http://www.ncbi.nlm.nih.gov/pubmed/24938289 %V 26

• Bauer, H., Ache, P., Lautner, S., Fromm, J., Hartung, W., Al-Rasheid, K.A.S., Sonnewald, S., Sonnewald, U., Kneitz, S., Lachmann, N., Mendel, R.R., Bittner, F., Hetherington, A.M., und Hedrich, R. (2013) „The stomatal response to reduced relative humidity requires guard cell-autonomous ABA synthesis“, Curr Biol, 23, 53–57, verfügbar unter: https://doi.org/10.1016/j.cub.2012.11.022.
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  @article{RN7, author = {Bauer, H. and Ache, P. and Lautner, S. and Fromm, J. and Hartung, W. and Al-Rasheid, K. A. S. and Sonnewald, S. and Sonnewald, U. and Kneitz, S. and Lachmann, N. and Mendel, R. R. and Bittner, F. and Hetherington, A. M. and Hedrich, R.}, journal = {Curr Biol}, keywords = {myown}, pages = {53-57}, title = {The stomatal response to reduced relative humidity requires guard cell-autonomous ABA synthesis}, type = {Journal Article}, volume = 23, year = 2013 }

  %0 Journal Article %1 RN7 %A Bauer, H. %A Ache, P. %A Lautner, S. %A Fromm, J. %A Hartung, W. %A Al-Rasheid, K. A. S. %A Sonnewald, S. %A Sonnewald, U. %A Kneitz, S. %A Lachmann, N. %A Mendel, R. R. %A Bittner, F. %A Hetherington, A. M. %A Hedrich, R. %D 2013 %J Curr Biol %P 53-57 %R 10.1016/j.cub.2012.11.022 %T The stomatal response to reduced relative humidity requires guard cell-autonomous ABA synthesis %U http://www.ncbi.nlm.nih.gov/pubmed/23219726 %V 23
• Bauer, H., Ache, P., Wohlfart, F., Al-Rasheid, K.A.S., Sonnewald, S., Sonnewald, U., Kneitz, S., Hetherington, A.M., und Hedrich, R. (2013) „How do stomata sense reductions in atmospheric relative humidity?“, Mol Plant, 6, 1703–1706, verfügbar unter: https://doi.org/10.1093/mp/sst055.
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  @article{RN8, author = {Bauer, H. and Ache, P. and Wohlfart, F. and Al-Rasheid, K. A. S. and Sonnewald, S. and Sonnewald, U. and Kneitz, S. and Hetherington, A. M. and Hedrich, R.}, journal = {Mol Plant}, keywords = {myown}, pages = {1703–1706}, title = {How do stomata sense reductions in atmospheric relative humidity?}, type = {Journal Article}, volume = 6, year = 2013 }

  %0 Journal Article %1 RN8 %A Bauer, H. %A Ache, P. %A Wohlfart, F. %A Al-Rasheid, K. A. S. %A Sonnewald, S. %A Sonnewald, U. %A Kneitz, S. %A Hetherington, A. M. %A Hedrich, R. %D 2013 %J Mol Plant %P 1703–1706 %R 10.1093/mp/sst055 %T How do stomata sense reductions in atmospheric relative humidity? %U http://www.ncbi.nlm.nih.gov/pubmed/23536729 %V 6

• Escalante-Perez, M., Jaborsky, M., Reinders, J., Kurzai, O., Hedrich, R., und Ache, P. (2012) „Poplar extrafloral nectar is protected against plant and human pathogenic fungus“, Mol Plant, 5, 1157–9, verfügbar unter: https://doi.org/10.1093/mp/sss072.
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  @article{RN17, author = {Escalante-Perez, M. and Jaborsky, M. and Reinders, J. and Kurzai, O. and Hedrich, R. and Ache, P.}, journal = {Mol Plant}, keywords = {myown}, pages = {1157-9}, title = {Poplar extrafloral nectar is protected against plant and human pathogenic fungus}, type = {Journal Article}, volume = 5, year = 2012 }

  %0 Journal Article %1 RN17 %A Escalante-Perez, M. %A Jaborsky, M. %A Reinders, J. %A Kurzai, O. %A Hedrich, R. %A Ache, P. %D 2012 %J Mol Plant %P 1157-9 %R 10.1093/mp/sss072 %T Poplar extrafloral nectar is protected against plant and human pathogenic fungus %U http://www.ncbi.nlm.nih.gov/pubmed/22859733 %V 5
• Larisch, C., Dittrich, M., Wildhagen, H., Lautner, S., Fromm, J., Polle, A., Hedrich, R., Rennenberg, H., Muller, T., und Ache, P. (2012) „Poplar wood rays are involved in seasonal remodeling of tree physiology“, Plant Physiol, 160, 1515–29, verfügbar unter: https://doi.org/10.1104/pp.112.202291.
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  @article{RN28, author = {Larisch, C. and Dittrich, M. and Wildhagen, H. and Lautner, S. and Fromm, J. and Polle, A. and Hedrich, R. and Rennenberg, H. and Muller, T. and Ache, P.}, journal = {Plant Physiol}, keywords = {myown}, pages = {1515-29}, title = {Poplar wood rays are involved in seasonal remodeling of tree physiology}, type = {Journal Article}, volume = 160, year = 2012 }

  %0 Journal Article %1 RN28 %A Larisch, C. %A Dittrich, M. %A Wildhagen, H. %A Lautner, S. %A Fromm, J. %A Polle, A. %A Hedrich, R. %A Rennenberg, H. %A Muller, T. %A Ache, P. %D 2012 %J Plant Physiol %P 1515-29 %R 10.1104/pp.112.202291 %T Poplar wood rays are involved in seasonal remodeling of tree physiology %U http://www.ncbi.nlm.nih.gov/pubmed/22992511 %V 160
• Escalante-Perez, M., Jaborsky, M., Lautner, S., Fromm, J., Muller, T., Dittrich, M., Kunert, M., Boland, W., Hedrich, R., und Ache, P. (2012) „Poplar extrafloral nectaries: two types, two strategies of indirect defenses against herbivores“, Plant Physiol, 159, 1176–91, verfügbar unter: https://doi.org/10.1104/pp.112.196014.
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  @article{RN16, author = {Escalante-Perez, M. and Jaborsky, M. and Lautner, S. and Fromm, J. and Muller, T. and Dittrich, M. and Kunert, M. and Boland, W. and Hedrich, R. and Ache, P.}, journal = {Plant Physiol}, keywords = {myown}, pages = {1176-91}, title = {Poplar extrafloral nectaries: two types, two strategies of indirect defenses against herbivores}, type = {Journal Article}, volume = 159, year = 2012 }

  %0 Journal Article %1 RN16 %A Escalante-Perez, M. %A Jaborsky, M. %A Lautner, S. %A Fromm, J. %A Muller, T. %A Dittrich, M. %A Kunert, M. %A Boland, W. %A Hedrich, R. %A Ache, P. %D 2012 %J Plant Physiol %P 1176-91 %R 10.1104/pp.112.196014 %T Poplar extrafloral nectaries: two types, two strategies of indirect defenses against herbivores %U http://www.plantphysiol.org/content/159/3/1176.full.pdf %V 159

• Geiger, D., Maierhofer, T., Al-Rasheid, K.A.S., Scherzer, S., Mumm, P., Liese, A., Ache, P., Wellmann, C., Marten, I., Grill, E., Romeis, T., und Hedrich, R. (2011) „Stomatal closure by fast abscisic acid signaling is mediated by the guard cell anion channel SLAH3 and the receptor RCAR1“, Sci Signal, 4, ra32, verfügbar unter: https://doi.org/10.1126/scisignal.2001346.
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  @article{RN19, author = {Geiger, D. and Maierhofer, T. and Al-Rasheid, K. A. S. and Scherzer, S. and Mumm, P. and Liese, A. and Ache, P. and Wellmann, C. and Marten, I. and Grill, E. and Romeis, T. and Hedrich, R.}, journal = {Sci Signal}, keywords = {myown}, pages = {ra32}, title = {Stomatal closure by fast abscisic acid signaling is mediated by the guard cell anion channel SLAH3 and the receptor RCAR1}, type = {Journal Article}, volume = 4, year = 2011 }

  %0 Journal Article %1 RN19 %A Geiger, D. %A Maierhofer, T. %A Al-Rasheid, K. A. S. %A Scherzer, S. %A Mumm, P. %A Liese, A. %A Ache, P. %A Wellmann, C. %A Marten, I. %A Grill, E. %A Romeis, T. %A Hedrich, R. %D 2011 %J Sci Signal %P ra32 %R 10.1126/scisignal.2001346 %T Stomatal closure by fast abscisic acid signaling is mediated by the guard cell anion channel SLAH3 and the receptor RCAR1 %U http://www.ncbi.nlm.nih.gov/pubmed/21586729 %V 4

• Ache, P., Bauer, H., Kollist, H., Al-Rasheid, K.A.S., Lautner, S., Hartung, W., und Hedrich, R. (2010) „Stomatal action directly feeds back on leaf turgor: new insights into the regulation of the plant water status from non-invasive pressure probe measurements“, Plant J, 62, 1072–82, verfügbar unter: https://doi.org/10.1111/j.1365-313X.2010.04213.x.
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  @article{RN1, author = {Ache, P. and Bauer, H. and Kollist, H. and Al-Rasheid, K. A. S. and Lautner, S. and Hartung, W. and Hedrich, R.}, journal = {Plant J}, keywords = {myown}, pages = {1072-82}, title = {Stomatal action directly feeds back on leaf turgor: new insights into the regulation of the plant water status from non-invasive pressure probe measurements}, type = {Journal Article}, volume = 62, year = 2010 }

  %0 Journal Article %1 RN1 %A Ache, P. %A Bauer, H. %A Kollist, H. %A Al-Rasheid, K. A. S. %A Lautner, S. %A Hartung, W. %A Hedrich, R. %D 2010 %J Plant J %P 1072-82 %R 10.1111/j.1365-313X.2010.04213.x %T Stomatal action directly feeds back on leaf turgor: new insights into the regulation of the plant water status from non-invasive pressure probe measurements %U http://www.ncbi.nlm.nih.gov/pubmed/20345603 %V 62
• Geiger, D., Scherzer, S., Mumm, P., Marten, I., Ache, P., Matschi, S., Liese, A., Wellmann, C., Al-Rasheid, K.A.S., Grill, E., Romeis, T., und Hedrich, R. (2010) „Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities“, Proc Natl Acad Sci U S A, 107, 8023–8, verfügbar unter: https://doi.org/10.1073/pnas.0912030107.
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  @article{RN20, author = {Geiger, D. and Scherzer, S. and Mumm, P. and Marten, I. and Ache, P. and Matschi, S. and Liese, A. and Wellmann, C. and Al-Rasheid, K. A. S. and Grill, E. and Romeis, T. and Hedrich, R.}, journal = {Proc Natl Acad Sci U S A}, keywords = {myown}, pages = {8023-8}, title = {Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities}, type = {Journal Article}, volume = 107, year = 2010 }

  %0 Journal Article %1 RN20 %A Geiger, D. %A Scherzer, S. %A Mumm, P. %A Marten, I. %A Ache, P. %A Matschi, S. %A Liese, A. %A Wellmann, C. %A Al-Rasheid, K. A. S. %A Grill, E. %A Romeis, T. %A Hedrich, R. %D 2010 %J Proc Natl Acad Sci U S A %P 8023-8 %R 10.1073/pnas.0912030107 %T Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities %U http://www.ncbi.nlm.nih.gov/pubmed/20385816 %V 107
• Herschbach, C., Teuber, M., Eiblmeier, M., Ehlting, B., Ache, P., Polle, A., Schnitzler, J.P., und Rennenberg, H. (2010) „Changes in sulphur metabolism of grey poplar (Populus x canescens) leaves during salt stress: a metabolic link to photorespiration“, Tree Physiol, 30, 1161–1173, verfügbar unter: https://doi.org/10.1093/treephys/tpq041.
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  The poplar hybrid Populus x canescens (syn. Populus tremula x Populus alba) was subjected to salt stress by applying 75 mM NaCl for 2 weeks in hydroponic cultures. Decreasing maximum quantum yield (Fv/Fm) indicated damage of photosystem II (PS II), which was more pronounced under nitrate compared with ammonium nutrition. In vivo staining with diaminobenzidine showed no accumulation of H(2)O(2) in the leaf lamina; moreover, staining intensity even decreased. But at the leaf margins, development of necrotic tissue was associated with a strong accumulation of H(2)O(2). Glutathione (GSH) contents increased in response to NaCl stress in leaves but not in roots, the primary site of salt exposure. The increasing leaf GSH concentrations correlated with stress-induced decreases in transpiration and net CO(2) assimilation rates at light saturation. Enhanced rates of photorespiration could also be involved in preventing reactive oxygen species formation in chloroplasts and, thus, in protecting PS II from damage. Accumulation of Gly and Ser in leaves indeed indicates increasing rates of photorespiration. Since Ser and Gly are both immediate precursors of GSH that can limit GSH synthesis, it is concluded that the salt-induced accumulation of leaf GSH results from enhanced photorespiration and is thus probably restricted to the cytosol.

  @article{Herschbach:2010:Tree-Physiol:20516486, abstract = {The poplar hybrid Populus x canescens (syn. Populus tremula x Populus alba) was subjected to salt stress by applying 75 mM NaCl for 2 weeks in hydroponic cultures. Decreasing maximum quantum yield (Fv/Fm) indicated damage of photosystem II (PS II), which was more pronounced under nitrate compared with ammonium nutrition. In vivo staining with diaminobenzidine showed no accumulation of H(2)O(2) in the leaf lamina; moreover, staining intensity even decreased. But at the leaf margins, development of necrotic tissue was associated with a strong accumulation of H(2)O(2). Glutathione (GSH) contents increased in response to NaCl stress in leaves but not in roots, the primary site of salt exposure. The increasing leaf GSH concentrations correlated with stress-induced decreases in transpiration and net CO(2) assimilation rates at light saturation. Enhanced rates of photorespiration could also be involved in preventing reactive oxygen species formation in chloroplasts and, thus, in protecting PS II from damage. Accumulation of Gly and Ser in leaves indeed indicates increasing rates of photorespiration. Since Ser and Gly are both immediate precursors of GSH that can limit GSH synthesis, it is concluded that the salt-induced accumulation of leaf GSH results from enhanced photorespiration and is thus probably restricted to the cytosol.}, author = {Herschbach, C and Teuber, M and Eiblmeier, M and Ehlting, B and Ache, P and Polle, A and Schnitzler, J P and Rennenberg, H}, journal = {Tree Physiol}, keywords = {myown}, month = {09}, pages = {1161-1173}, title = {Changes in sulphur metabolism of grey poplar (Populus x canescens) leaves during salt stress: a metabolic link to photorespiration}, volume = 30, year = 2010 }

  %0 Journal Article %1 Herschbach:2010:Tree-Physiol:20516486 %A Herschbach, C %A Teuber, M %A Eiblmeier, M %A Ehlting, B %A Ache, P %A Polle, A %A Schnitzler, J P %A Rennenberg, H %D 2010 %J Tree Physiol %P 1161-1173 %R 10.1093/treephys/tpq041 %T Changes in sulphur metabolism of grey poplar (Populus x canescens) leaves during salt stress: a metabolic link to photorespiration %U https://www.ncbi.nlm.nih.gov/pubmed/20516486 %V 30 %X The poplar hybrid Populus x canescens (syn. Populus tremula x Populus alba) was subjected to salt stress by applying 75 mM NaCl for 2 weeks in hydroponic cultures. Decreasing maximum quantum yield (Fv/Fm) indicated damage of photosystem II (PS II), which was more pronounced under nitrate compared with ammonium nutrition. In vivo staining with diaminobenzidine showed no accumulation of H(2)O(2) in the leaf lamina; moreover, staining intensity even decreased. But at the leaf margins, development of necrotic tissue was associated with a strong accumulation of H(2)O(2). Glutathione (GSH) contents increased in response to NaCl stress in leaves but not in roots, the primary site of salt exposure. The increasing leaf GSH concentrations correlated with stress-induced decreases in transpiration and net CO(2) assimilation rates at light saturation. Enhanced rates of photorespiration could also be involved in preventing reactive oxygen species formation in chloroplasts and, thus, in protecting PS II from damage. Accumulation of Gly and Ser in leaves indeed indicates increasing rates of photorespiration. Since Ser and Gly are both immediate precursors of GSH that can limit GSH synthesis, it is concluded that the salt-induced accumulation of leaf GSH results from enhanced photorespiration and is thus probably restricted to the cytosol.
• Ache, P., Fromm, J., und Hedrich, R. (2010) „Potassium-dependent wood formation in poplar: seasonal aspects and environmental limitations“, Plant Biol, 12, 259–67, verfügbar unter: https://doi.org/10.1111/j.1438-8677.2009.00282.x.
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  @article{RN4, author = {Ache, P. and Fromm, J. and Hedrich, R.}, journal = {Plant Biol}, keywords = {myown}, pages = {259-67}, title = {Potassium-dependent wood formation in poplar: seasonal aspects and environmental limitations}, type = {Publication}, volume = 12, year = 2010 }

  %0 Journal Article %1 RN4 %A Ache, P. %A Fromm, J. %A Hedrich, R. %D 2010 %J Plant Biol %P 259-67 %R 10.1111/j.1438-8677.2009.00282.x %T Potassium-dependent wood formation in poplar: seasonal aspects and environmental limitations %U http://www.ncbi.nlm.nih.gov/pubmed/20398233 %V 12
• Rienmuller, F., Beyhl, D., Lautner, S., Fromm, J., Al-Rasheid, K.A.S., Ache, P., Farmer, E.E., Marten, I., und Hedrich, R. (2010) „Guard cell-specific calcium sensitivity of high density and activity SV/TPC1 channels“, Plant Cell Physiol, 51, 1548–54, verfügbar unter: https://doi.org/10.1093/pcp/pcq102.
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  @article{RN35, author = {Rienmuller, F. and Beyhl, D. and Lautner, S. and Fromm, J. and Al-Rasheid, K. A. S. and Ache, P. and Farmer, E. E. and Marten, I. and Hedrich, R.}, journal = {Plant Cell Physiol}, keywords = {myown}, pages = {1548-54}, title = {Guard cell-specific calcium sensitivity of high density and activity SV/TPC1 channels}, type = {Journal Article}, volume = 51, year = 2010 }

  %0 Journal Article %1 RN35 %A Rienmuller, F. %A Beyhl, D. %A Lautner, S. %A Fromm, J. %A Al-Rasheid, K. A. S. %A Ache, P. %A Farmer, E. E. %A Marten, I. %A Hedrich, R. %D 2010 %J Plant Cell Physiol %P 1548-54 %R 10.1093/pcp/pcq102 %T Guard cell-specific calcium sensitivity of high density and activity SV/TPC1 channels %U http://www.ncbi.nlm.nih.gov/pubmed/20630987 %V 51

• Escalante-Perez, M., Lautner, S., Nehls, U., Selle, A., Teuber, M., Schnitzler, J.P., Teichmann, T., Fayyaz, P., Hartung, W., Polle, A., Fromm, J., Hedrich, R., und Ache, P. (2009) „Salt stress affects xylem differentiation of grey poplar (Populus x canescens)“, Planta, 229, 299–309, verfügbar unter: https://doi.org/10.1007/s00425-008-0829-7.
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  @article{RN18, author = {Escalante-Perez, M. and Lautner, S. and Nehls, U. and Selle, A. and Teuber, M. and Schnitzler, J. P. and Teichmann, T. and Fayyaz, P. and Hartung, W. and Polle, A. and Fromm, J. and Hedrich, R. and Ache, P.}, journal = {Planta}, keywords = {myown}, pages = {299-309}, title = {Salt stress affects xylem differentiation of grey poplar (Populus x canescens)}, type = {Journal Article}, volume = 229, year = 2009 }

  %0 Journal Article %1 RN18 %A Escalante-Perez, M. %A Lautner, S. %A Nehls, U. %A Selle, A. %A Teuber, M. %A Schnitzler, J. P. %A Teichmann, T. %A Fayyaz, P. %A Hartung, W. %A Polle, A. %A Fromm, J. %A Hedrich, R. %A Ache, P. %D 2009 %J Planta %P 299-309 %R 10.1007/s00425-008-0829-7 %T Salt stress affects xylem differentiation of grey poplar (Populus x canescens) %U http://www.ncbi.nlm.nih.gov/pubmed/18946679 %V 229
• Geiger, D., Scherzer, S., Mumm, P., Stange, A., Marten, I., Bauer, H., Ache, P., Matschi, S., Liese, A., Al-Rasheid, K.A.S., Romeis, T., und Hedrich, R. (2009) „Activity of guard cell anion channel SLAC1 is controlled by drought-stress signaling kinase-phosphatase pair“, Proc Natl Acad Sci U S A, 106, 21425–30, verfügbar unter: https://doi.org/10.1073/pnas.0912021106.
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  @article{RN21, author = {Geiger, D. and Scherzer, S. and Mumm, P. and Stange, A. and Marten, I. and Bauer, H. and Ache, P. and Matschi, S. and Liese, A. and Al-Rasheid, K. A. S. and Romeis, T. and Hedrich, R.}, journal = {Proc Natl Acad Sci U S A}, keywords = {myown}, pages = {21425-30}, title = {Activity of guard cell anion channel SLAC1 is controlled by drought-stress signaling kinase-phosphatase pair}, type = {Journal Article}, volume = 106, year = 2009 }

  %0 Journal Article %1 RN21 %A Geiger, D. %A Scherzer, S. %A Mumm, P. %A Stange, A. %A Marten, I. %A Bauer, H. %A Ache, P. %A Matschi, S. %A Liese, A. %A Al-Rasheid, K. A. S. %A Romeis, T. %A Hedrich, R. %D 2009 %J Proc Natl Acad Sci U S A %P 21425-30 %R 10.1073/pnas.0912021106 %T Activity of guard cell anion channel SLAC1 is controlled by drought-stress signaling kinase-phosphatase pair %U http://www.ncbi.nlm.nih.gov/pubmed/19955405 %V 106

• Teuber, M., Zimmer, I., Kreuzwieser, J., Ache, P., Polle, A., Rennenberg, H., und Schnitzler, J.P. (2008) „VOC emissions of Grey poplar leaves as affected by salt stress and different N sources“, Plant Biol, 10, 86–96, verfügbar unter: https://doi.org/10.1111/j.1438-8677.2007.00015.x.
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  Nitrogen nutrition and salt stress experiments were performed in a greenhouse with hydroponic-cultured, salt-sensitive Grey poplar (Populus x canescens) plants to study the combined influence of different N sources (either 1 mm NO(3) (-) or NH(4)(+)) and salt (up to 75 mm NaCl) on leaf gas exchange, isoprene biosynthesis and VOC emissions. Net assimilation and transpiration proved to be highly sensitive to salt stress and were reduced by approximately 90% at leaf sodium concentrations higher than 1,800 microg Na g dry weight (dw)(-1). In contrast, emissions of isoprene and oxygenated VOC (i.e. acetaldehyde, formaldehyde and acetone) were unaffected. There was no significant effect of combinations of salt stress and N source, and neither NO(3)(-) or NH(4)(+) influenced the salt stress response in the Grey poplar leaves. Also, transcript levels of 1-deoxy-d-xylulose 5-phosphate reductoisomerase (PcDXR) and isoprene synthase (PcISPS) did not respond to the different N sources and only responded slightly to salt application, although isoprene synthase (PcISPS) activity was negatively affected at least in one of two experiments, despite high isoprene emission rates. A significant salt effect was the strong reduction of leaf dimethylallyl diphosphate (DMADP) content, probably due to restricted availability of photosynthates for DMADP biosynthesis. Further consequences of reduced photosynthetic gas exchange and maintaining VOC emissions are a very high C loss, up to 50%, from VOC emissions related to net CO(2) uptake and a strong increase in leaf internal isoprene concentrations, with maximum mean values up to 6.6 microl x l(-1). Why poplar leaves maintain VOC biosynthesis and emission under salt stress conditions, despite impaired photosynthetic CO(2) fixation, is discussed.

  @article{Teuber:2008:Plant-Biol-Stuttg:18211549, abstract = {Nitrogen nutrition and salt stress experiments were performed in a greenhouse with hydroponic-cultured, salt-sensitive Grey poplar (Populus x canescens) plants to study the combined influence of different N sources (either 1 mm NO(3) (-) or NH(4)(+)) and salt (up to 75 mm NaCl) on leaf gas exchange, isoprene biosynthesis and VOC emissions. Net assimilation and transpiration proved to be highly sensitive to salt stress and were reduced by approximately 90% at leaf sodium concentrations higher than 1,800 microg Na g dry weight (dw)(-1). In contrast, emissions of isoprene and oxygenated VOC (i.e. acetaldehyde, formaldehyde and acetone) were unaffected. There was no significant effect of combinations of salt stress and N source, and neither NO(3)(-) or NH(4)(+) influenced the salt stress response in the Grey poplar leaves. Also, transcript levels of 1-deoxy-d-xylulose 5-phosphate reductoisomerase (PcDXR) and isoprene synthase (PcISPS) did not respond to the different N sources and only responded slightly to salt application, although isoprene synthase (PcISPS) activity was negatively affected at least in one of two experiments, despite high isoprene emission rates. A significant salt effect was the strong reduction of leaf dimethylallyl diphosphate (DMADP) content, probably due to restricted availability of photosynthates for DMADP biosynthesis. Further consequences of reduced photosynthetic gas exchange and maintaining VOC emissions are a very high C loss, up to 50%, from VOC emissions related to net CO(2) uptake and a strong increase in leaf internal isoprene concentrations, with maximum mean values up to 6.6 microl x l(-1). Why poplar leaves maintain VOC biosynthesis and emission under salt stress conditions, despite impaired photosynthetic CO(2) fixation, is discussed.}, author = {Teuber, M and Zimmer, I and Kreuzwieser, J and Ache, P and Polle, A and Rennenberg, H and Schnitzler, J P}, journal = {Plant Biol}, keywords = {myown}, month = {01}, pages = {86-96}, title = {VOC emissions of Grey poplar leaves as affected by salt stress and different N sources}, volume = 10, year = 2008 }

  %0 Journal Article %1 Teuber:2008:Plant-Biol-Stuttg:18211549 %A Teuber, M %A Zimmer, I %A Kreuzwieser, J %A Ache, P %A Polle, A %A Rennenberg, H %A Schnitzler, J P %D 2008 %J Plant Biol %P 86-96 %R 10.1111/j.1438-8677.2007.00015.x %T VOC emissions of Grey poplar leaves as affected by salt stress and different N sources %U https://www.ncbi.nlm.nih.gov/pubmed/18211549 %V 10 %X Nitrogen nutrition and salt stress experiments were performed in a greenhouse with hydroponic-cultured, salt-sensitive Grey poplar (Populus x canescens) plants to study the combined influence of different N sources (either 1 mm NO(3) (-) or NH(4)(+)) and salt (up to 75 mm NaCl) on leaf gas exchange, isoprene biosynthesis and VOC emissions. Net assimilation and transpiration proved to be highly sensitive to salt stress and were reduced by approximately 90% at leaf sodium concentrations higher than 1,800 microg Na g dry weight (dw)(-1). In contrast, emissions of isoprene and oxygenated VOC (i.e. acetaldehyde, formaldehyde and acetone) were unaffected. There was no significant effect of combinations of salt stress and N source, and neither NO(3)(-) or NH(4)(+) influenced the salt stress response in the Grey poplar leaves. Also, transcript levels of 1-deoxy-d-xylulose 5-phosphate reductoisomerase (PcDXR) and isoprene synthase (PcISPS) did not respond to the different N sources and only responded slightly to salt application, although isoprene synthase (PcISPS) activity was negatively affected at least in one of two experiments, despite high isoprene emission rates. A significant salt effect was the strong reduction of leaf dimethylallyl diphosphate (DMADP) content, probably due to restricted availability of photosynthates for DMADP biosynthesis. Further consequences of reduced photosynthetic gas exchange and maintaining VOC emissions are a very high C loss, up to 50%, from VOC emissions related to net CO(2) uptake and a strong increase in leaf internal isoprene concentrations, with maximum mean values up to 6.6 microl x l(-1). Why poplar leaves maintain VOC biosynthesis and emission under salt stress conditions, despite impaired photosynthetic CO(2) fixation, is discussed.
• Deeken, R., Ache, P., Kajahn, I., Klinkenberg, J., Bringmann, G., und Hedrich, R. (2008) „Identification of Arabidopsis thaliana phloem RNAs provides a search criterion for phloem-based transcripts hidden in complex datasets of microarray experiments“, Plant J, 55, 746–59, verfügbar unter: https://doi.org/10.1111/j.1365-313X.2008.03555.x.
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  @article{RN11, author = {Deeken, R. and Ache, P. and Kajahn, I. and Klinkenberg, J. and Bringmann, G. and Hedrich, R.}, journal = {Plant J}, keywords = {myown}, pages = {746-59}, title = {Identification of Arabidopsis thaliana phloem RNAs provides a search criterion for phloem-based transcripts hidden in complex datasets of microarray experiments}, type = {Journal Article}, volume = 55, year = 2008 }

  %0 Journal Article %1 RN11 %A Deeken, R. %A Ache, P. %A Kajahn, I. %A Klinkenberg, J. %A Bringmann, G. %A Hedrich, R. %D 2008 %J Plant J %P 746-59 %R 10.1111/j.1365-313X.2008.03555.x %T Identification of Arabidopsis thaliana phloem RNAs provides a search criterion for phloem-based transcripts hidden in complex datasets of microarray experiments %U http://www.ncbi.nlm.nih.gov/pubmed/18485061 %V 55

• Latz, A., Ivashikina, N., Fischer, S., Ache, P., Sano, T., Becker, D., Deeken, R., und Hedrich, R. (2007) „In planta AKT2 subunits constitute a pH- and Ca2+-sensitive inward rectifying K+ channel“, Planta, 225, 1179–91, verfügbar unter: https://doi.org/10.1007/s00425-006-0428-4.
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  @article{RN29, author = {Latz, A. and Ivashikina, N. and Fischer, S. and Ache, P. and Sano, T. and Becker, D. and Deeken, R. and Hedrich, R.}, journal = {Planta}, keywords = {myown}, pages = {1179-91}, title = {In planta AKT2 subunits constitute a pH- and Ca2+-sensitive inward rectifying K+ channel}, type = {Journal Article}, volume = 225, year = 2007 }

  %0 Journal Article %1 RN29 %A Latz, A. %A Ivashikina, N. %A Fischer, S. %A Ache, P. %A Sano, T. %A Becker, D. %A Deeken, R. %A Hedrich, R. %D 2007 %J Planta %P 1179-91 %R 10.1007/s00425-006-0428-4 %T In planta AKT2 subunits constitute a pH- and Ca2+-sensitive inward rectifying K+ channel %U http://www.ncbi.nlm.nih.gov/pubmed/17146665 %V 225
• Zimmermann, D., Westhoff, M., Zimmermann, G., Gessner, P., Gessner, A., Wegner, L.H., Rokitta, M., Ache, P., Schneider, H., Vasquez, J.A., Kruck, W., Shirley, S., Jakob, P., Hedrich, R., Bentrup, F.W., Bamberg, E., und Zimmermann, U. (2007) „Foliar water supply of tall trees: evidence for mucilage-facilitated moisture uptake from the atmosphere and the impact on pressure bomb measurements“, Protoplasma, 232, 11–34, verfügbar unter: https://doi.org/10.1007/s00709-007-0279-2.
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  @article{RN39, author = {Zimmermann, D. and Westhoff, M. and Zimmermann, G. and Gessner, P. and Gessner, A. and Wegner, L. H. and Rokitta, M. and Ache, P. and Schneider, H. and Vasquez, J. A. and Kruck, W. and Shirley, S. and Jakob, P. and Hedrich, R. and Bentrup, F. W. and Bamberg, E. and Zimmermann, U.}, journal = {Protoplasma}, keywords = {myown}, pages = {11-34}, title = {Foliar water supply of tall trees: evidence for mucilage-facilitated moisture uptake from the atmosphere and the impact on pressure bomb measurements}, type = {Journal Article}, volume = 232, year = 2007 }

  %0 Journal Article %1 RN39 %A Zimmermann, D. %A Westhoff, M. %A Zimmermann, G. %A Gessner, P. %A Gessner, A. %A Wegner, L. H. %A Rokitta, M. %A Ache, P. %A Schneider, H. %A Vasquez, J. A. %A Kruck, W. %A Shirley, S. %A Jakob, P. %A Hedrich, R. %A Bentrup, F. W. %A Bamberg, E. %A Zimmermann, U. %D 2007 %J Protoplasma %P 11-34 %R 10.1007/s00709-007-0279-2 %T Foliar water supply of tall trees: evidence for mucilage-facilitated moisture uptake from the atmosphere and the impact on pressure bomb measurements %U http://www.ncbi.nlm.nih.gov/pubmed/18176835 %V 232

• Niewiadomski, P., Knappe, S., Geimer, S., Fischer, K., Schulz, B., Unte, U.S., Rosso, M.G., Ache, P., Fl{ü}gge, U.I., und Schneider, A. (2005) „The Arabidopsis plastidic glucose 6-phosphate/phosphate translocator GPT1 is essential for pollen maturation and embryo sac development“, Plant Cell, 17, 760–775, verfügbar unter: https://doi.org/10.1105/tpc.104.029124.
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  Plastids of nongreen tissues can import carbon in the form of glucose 6-phosphate via the glucose 6-phosphate/phosphate translocator (GPT). The Arabidopsis thaliana genome contains two homologous GPT genes, AtGPT1 and AtGPT2. Both proteins show glucose 6-phosphate translocator activity after reconstitution in liposomes, and each of them can rescue the low-starch leaf phenotype of the pgi1 mutant (which lacks plastid phosphoglucoisomerase), indicating that the two proteins are also functional in planta. AtGPT1 transcripts are ubiquitously expressed during plant development, with highest expression in stamens, whereas AtGPT2 expression is restricted to a few tissues, including senescing leaves. Disruption of GPT2 has no obvious effect on growth and development under greenhouse conditions, whereas the mutations gpt1-1 and gpt1-2 are lethal. In both gpt1 lines, distorted segregation ratios, reduced efficiency of transmission in males and females, and inability to complete pollen and ovule development were observed, indicating profound defects in gametogenesis. Embryo sac development is arrested in the gpt1 mutants at a stage before the fusion of the polar nuclei. Mutant pollen development is associated with reduced formation of lipid bodies and small vesicles and the disappearance of dispersed vacuoles, which results in disintegration of the pollen structure. Taken together, our results indicate that GPT1-mediated import of glucose 6-phosphate into nongreen plastids is crucial for gametophyte development. We suggest that loss of GPT1 function results in disruption of the oxidative pentose phosphate cycle, which in turn affects fatty acid biosynthesis.

  @article{Niewiadomski:2005:Plant-Cell:15722468, abstract = {Plastids of nongreen tissues can import carbon in the form of glucose 6-phosphate via the glucose 6-phosphate/phosphate translocator (GPT). The Arabidopsis thaliana genome contains two homologous GPT genes, AtGPT1 and AtGPT2. Both proteins show glucose 6-phosphate translocator activity after reconstitution in liposomes, and each of them can rescue the low-starch leaf phenotype of the pgi1 mutant (which lacks plastid phosphoglucoisomerase), indicating that the two proteins are also functional in planta. AtGPT1 transcripts are ubiquitously expressed during plant development, with highest expression in stamens, whereas AtGPT2 expression is restricted to a few tissues, including senescing leaves. Disruption of GPT2 has no obvious effect on growth and development under greenhouse conditions, whereas the mutations gpt1-1 and gpt1-2 are lethal. In both gpt1 lines, distorted segregation ratios, reduced efficiency of transmission in males and females, and inability to complete pollen and ovule development were observed, indicating profound defects in gametogenesis. Embryo sac development is arrested in the gpt1 mutants at a stage before the fusion of the polar nuclei. Mutant pollen development is associated with reduced formation of lipid bodies and small vesicles and the disappearance of dispersed vacuoles, which results in disintegration of the pollen structure. Taken together, our results indicate that GPT1-mediated import of glucose 6-phosphate into nongreen plastids is crucial for gametophyte development. We suggest that loss of GPT1 function results in disruption of the oxidative pentose phosphate cycle, which in turn affects fatty acid biosynthesis.}, author = {Niewiadomski, P and Knappe, S and Geimer, S and Fischer, K and Schulz, B and Unte, U S and Rosso, M G and Ache, P and Fl{\"u}gge, U I and Schneider, A}, journal = {Plant Cell}, keywords = {myown}, month = {03}, pages = {760-775}, title = {The Arabidopsis plastidic glucose 6-phosphate/phosphate translocator GPT1 is essential for pollen maturation and embryo sac development}, volume = 17, year = 2005 }

  %0 Journal Article %1 Niewiadomski:2005:Plant-Cell:15722468 %A Niewiadomski, P %A Knappe, S %A Geimer, S %A Fischer, K %A Schulz, B %A Unte, U S %A Rosso, M G %A Ache, P %A Fl{ü}gge, U I %A Schneider, A %D 2005 %J Plant Cell %P 760-775 %R 10.1105/tpc.104.029124 %T The Arabidopsis plastidic glucose 6-phosphate/phosphate translocator GPT1 is essential for pollen maturation and embryo sac development %U https://www.ncbi.nlm.nih.gov/pubmed/15722468 %V 17 %X Plastids of nongreen tissues can import carbon in the form of glucose 6-phosphate via the glucose 6-phosphate/phosphate translocator (GPT). The Arabidopsis thaliana genome contains two homologous GPT genes, AtGPT1 and AtGPT2. Both proteins show glucose 6-phosphate translocator activity after reconstitution in liposomes, and each of them can rescue the low-starch leaf phenotype of the pgi1 mutant (which lacks plastid phosphoglucoisomerase), indicating that the two proteins are also functional in planta. AtGPT1 transcripts are ubiquitously expressed during plant development, with highest expression in stamens, whereas AtGPT2 expression is restricted to a few tissues, including senescing leaves. Disruption of GPT2 has no obvious effect on growth and development under greenhouse conditions, whereas the mutations gpt1-1 and gpt1-2 are lethal. In both gpt1 lines, distorted segregation ratios, reduced efficiency of transmission in males and females, and inability to complete pollen and ovule development were observed, indicating profound defects in gametogenesis. Embryo sac development is arrested in the gpt1 mutants at a stage before the fusion of the polar nuclei. Mutant pollen development is associated with reduced formation of lipid bodies and small vesicles and the disappearance of dispersed vacuoles, which results in disintegration of the pollen structure. Taken together, our results indicate that GPT1-mediated import of glucose 6-phosphate into nongreen plastids is crucial for gametophyte development. We suggest that loss of GPT1 function results in disruption of the oxidative pentose phosphate cycle, which in turn affects fatty acid biosynthesis.
• Buchsenschutz, K., Marten, I., Becker, D., Philippar, K., Ache, P., und Hedrich, R. (2005) „Differential expression of K+ channels between guard cells and subsidiary cells within the maize stomatal complex“, Planta, 222, 968–76, verfügbar unter: https://doi.org/10.1007/s00425-005-0038-6.
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  @article{RN10, author = {Buchsenschutz, K. and Marten, I. and Becker, D. and Philippar, K. and Ache, P. and Hedrich, R.}, journal = {Planta}, keywords = {myown}, pages = {968-76}, title = {Differential expression of K+ channels between guard cells and subsidiary cells within the maize stomatal complex}, type = {Journal Article}, volume = 222, year = 2005 }

  %0 Journal Article %1 RN10 %A Buchsenschutz, K. %A Marten, I. %A Becker, D. %A Philippar, K. %A Ache, P. %A Hedrich, R. %D 2005 %J Planta %P 968-76 %R 10.1007/s00425-005-0038-6 %T Differential expression of K+ channels between guard cells and subsidiary cells within the maize stomatal complex %U http://www.ncbi.nlm.nih.gov/pubmed/16021501 %V 222
• Ivashikina, N., Deeken, R., Fischer, S., Ache, P., und Hedrich, R. (2005) „AKT2/3 subunits render guard cell K+ channels Ca2+ sensitive“, J Gen Physiol, 125, 483–92, verfügbar unter: https://doi.org/10.1085/jgp.200409211.
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  @article{RN24, author = {Ivashikina, N. and Deeken, R. and Fischer, S. and Ache, P. and Hedrich, R.}, journal = {J Gen Physiol}, keywords = {myown}, pages = {483-92}, title = {AKT2/3 subunits render guard cell K+ channels Ca2+ sensitive}, type = {Journal Article}, volume = 125, year = 2005 }

  %0 Journal Article %1 RN24 %A Ivashikina, N. %A Deeken, R. %A Fischer, S. %A Ache, P. %A Hedrich, R. %D 2005 %J J Gen Physiol %P 483-92 %R 10.1085/jgp.200409211 %T AKT2/3 subunits render guard cell K+ channels Ca2+ sensitive %U http://www.ncbi.nlm.nih.gov/pubmed/15824192 %V 125
• Arend, M., Stinzing, A., Wind, C., Langer, K., Latz, A., Ache, P., Fromm, J., und Hedrich, R. (2005) „Polar-localised poplar K+ channel capable of controlling electrical properties of wood-forming cells“, Planta, 223, 140–8, verfügbar unter: https://doi.org/10.1007/s00425-005-0122-y.
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  @article{RN6, author = {Arend, M. and Stinzing, A. and Wind, C. and Langer, K. and Latz, A. and Ache, P. and Fromm, J. and Hedrich, R.}, journal = {Planta}, keywords = {myown}, pages = {140-8}, title = {Polar-localised poplar K+ channel capable of controlling electrical properties of wood-forming cells}, type = {Journal Article}, volume = 223, year = 2005 }

  %0 Journal Article %1 RN6 %A Arend, M. %A Stinzing, A. %A Wind, C. %A Langer, K. %A Latz, A. %A Ache, P. %A Fromm, J. %A Hedrich, R. %D 2005 %J Planta %P 140-8 %R 10.1007/s00425-005-0122-y %T Polar-localised poplar K+ channel capable of controlling electrical properties of wood-forming cells %U http://www.ncbi.nlm.nih.gov/pubmed/16258747 %V 223

• Langer, K., Levchenko, V., Fromm, J., Geiger, D., Steinmeyer, R., Lautner, S., Ache, P., und Hedrich, R. (2004) „The poplar K+ channel KPT1 is associated with K+ uptake during stomatal opening and bud development“, Plant J, 37, 828–38, verfügbar unter: http://www.ncbi.nlm.nih.gov/pubmed/14996212.
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  @article{RN27, author = {Langer, K. and Levchenko, V. and Fromm, J. and Geiger, D. and Steinmeyer, R. and Lautner, S. and Ache, P. and Hedrich, R.}, journal = {Plant J}, keywords = {myown}, pages = {828-38}, title = {The poplar K+ channel KPT1 is associated with K+ uptake during stomatal opening and bud development}, type = {Journal Article}, volume = 37, year = 2004 }

  %0 Journal Article %1 RN27 %A Langer, K. %A Levchenko, V. %A Fromm, J. %A Geiger, D. %A Steinmeyer, R. %A Lautner, S. %A Ache, P. %A Hedrich, R. %D 2004 %J Plant J %P 828-38 %T The poplar K+ channel KPT1 is associated with K+ uptake during stomatal opening and bud development %U http://www.ncbi.nlm.nih.gov/pubmed/14996212 %V 37
• Philippar, K., Ivashikina, N., Ache, P., Christian, M., Luthen, H., Palme, K., und Hedrich, R. (2004) „Auxin activates KAT1 and KAT2, two K+-channel genes expressed in seedlings of Arabidopsis thaliana“, Plant J, 37, 815–27, verfügbar unter: http://www.ncbi.nlm.nih.gov/pubmed/14996216.
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  @article{RN32, author = {Philippar, K. and Ivashikina, N. and Ache, P. and Christian, M. and Luthen, H. and Palme, K. and Hedrich, R.}, journal = {Plant J}, keywords = {myown}, pages = {815-27}, title = {Auxin activates KAT1 and KAT2, two K+-channel genes expressed in seedlings of Arabidopsis thaliana}, type = {Journal Article}, volume = 37, year = 2004 }

  %0 Journal Article %1 RN32 %A Philippar, K. %A Ivashikina, N. %A Ache, P. %A Christian, M. %A Luthen, H. %A Palme, K. %A Hedrich, R. %D 2004 %J Plant J %P 815-27 %T Auxin activates KAT1 and KAT2, two K+-channel genes expressed in seedlings of Arabidopsis thaliana %U http://www.ncbi.nlm.nih.gov/pubmed/14996216 %V 37

• Becker, D., Hoth, S., Ache, P., Wenkel, S., Roelfsema, M.R., Meyerhoff, O., Hartung, W., und Hedrich, R. (2003) „Regulation of the ABA-sensitive Arabidopsis potassium channel gene GORK in response to water stress“, FEBS Lett, 554, 119–26, verfügbar unter: https://www.ncbi.nlm.nih.gov/pubmed/14596925.
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  @article{RN9, author = {Becker, D. and Hoth, S. and Ache, P. and Wenkel, S. and Roelfsema, M. R. and Meyerhoff, O. and Hartung, W. and Hedrich, R.}, journal = {FEBS Lett}, keywords = {myown}, pages = {119-26}, title = {Regulation of the ABA-sensitive Arabidopsis potassium channel gene GORK in response to water stress}, type = {Journal Article}, volume = 554, year = 2003 }

  %0 Journal Article %1 RN9 %A Becker, D. %A Hoth, S. %A Ache, P. %A Wenkel, S. %A Roelfsema, M. R. %A Meyerhoff, O. %A Hartung, W. %A Hedrich, R. %D 2003 %J FEBS Lett %P 119-26 %T Regulation of the ABA-sensitive Arabidopsis potassium channel gene GORK in response to water stress %U https://www.ncbi.nlm.nih.gov/pubmed/14596925 %V 554
• Ivashikina, N., Deeken, R., Ache, P., Kranz, E., Pommerrenig, B., Sauer, N., und Hedrich, R. (2003) „Isolation of AtSUC2 promoter-GFP-marked companion cells for patch-clamp studies and expression profiling“, Plant J, 36, 931–45, verfügbar unter: http://www.ncbi.nlm.nih.gov/pubmed/14675456.
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  @article{RN23, author = {Ivashikina, N. and Deeken, R. and Ache, P. and Kranz, E. and Pommerrenig, B. and Sauer, N. and Hedrich, R.}, journal = {Plant J}, keywords = {myown}, pages = {931-45}, title = {Isolation of AtSUC2 promoter-GFP-marked companion cells for patch-clamp studies and expression profiling}, type = {Journal Article}, volume = 36, year = 2003 }

  %0 Journal Article %1 RN23 %A Ivashikina, N. %A Deeken, R. %A Ache, P. %A Kranz, E. %A Pommerrenig, B. %A Sauer, N. %A Hedrich, R. %D 2003 %J Plant J %P 931-45 %T Isolation of AtSUC2 promoter-GFP-marked companion cells for patch-clamp studies and expression profiling %U http://www.ncbi.nlm.nih.gov/pubmed/14675456 %V 36
• Stadler, R., Buttner, M., Ache, P., Hedrich, R., Ivashikina, N., Melzer, M., Shearson, S.M., Smith, S.M., und Sauer, N. (2003) „Diurnal and light-regulated expression of AtSTP1 in guard cells of Arabidopsis“, Plant Physol, 133, 528–37, verfügbar unter: https://doi.org/10.1104/pp.103.024240.
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  @article{RN37, author = {Stadler, R. and Buttner, M. and Ache, P. and Hedrich, R. and Ivashikina, N. and Melzer, M. and Shearson, S. M. and Smith, S. M. and Sauer, N.}, journal = {Plant Physol}, keywords = {myown}, pages = {528-37}, title = {Diurnal and light-regulated expression of AtSTP1 in guard cells of Arabidopsis}, type = {Journal Article}, volume = 133, year = 2003 }

  %0 Journal Article %1 RN37 %A Stadler, R. %A Buttner, M. %A Ache, P. %A Hedrich, R. %A Ivashikina, N. %A Melzer, M. %A Shearson, S. M. %A Smith, S. M. %A Sauer, N. %D 2003 %J Plant Physol %P 528-37 %R 10.1104/pp.103.024240 %T Diurnal and light-regulated expression of AtSTP1 in guard cells of Arabidopsis %U http://www.ncbi.nlm.nih.gov/pubmed/12972665 %V 133
• Deeken, R., Ivashikina, N., Czirjak, T., Philippar, K., Becker, D., Ache, P., und Hedrich, R. (2003) „Tumour development in Arabidopsis thaliana involves the Shaker-like K+ channels AKT1 and AKT2/3“, Plant J, 34, 778–87, verfügbar unter: http://www.ncbi.nlm.nih.gov/pubmed/12795698.
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  @article{RN13, author = {Deeken, R. and Ivashikina, N. and Czirjak, T. and Philippar, K. and Becker, D. and Ache, P. and Hedrich, R.}, journal = {Plant J}, keywords = {myown}, pages = {778-87}, title = {Tumour development in Arabidopsis thaliana involves the Shaker-like K+ channels AKT1 and AKT2/3}, type = {Journal Article}, volume = 34, year = 2003 }

  %0 Journal Article %1 RN13 %A Deeken, R. %A Ivashikina, N. %A Czirjak, T. %A Philippar, K. %A Becker, D. %A Ache, P. %A Hedrich, R. %D 2003 %J Plant J %P 778-87 %T Tumour development in Arabidopsis thaliana involves the Shaker-like K+ channels AKT1 and AKT2/3 %U http://www.ncbi.nlm.nih.gov/pubmed/12795698 %V 34

• Langer, K., Ache, P., Geiger, D., Stinzing, A., Arend, M., Wind, C., Regan, S., Fromm, J., und Hedrich, R. (2002) „Poplar potassium transporters capable of controlling K+ homeostasis and K+-dependent xylogenesis“, Plant J, 32, 997–1009, verfügbar unter: http://www.ncbi.nlm.nih.gov/pubmed/12492841.
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  @article{RN26, author = {Langer, K. and Ache, P. and Geiger, D. and Stinzing, A. and Arend, M. and Wind, C. and Regan, S. and Fromm, J. and Hedrich, R.}, journal = {Plant J}, keywords = {myown}, pages = {997-1009}, title = {Poplar potassium transporters capable of controlling K+ homeostasis and K+-dependent xylogenesis}, type = {Journal Article}, volume = 32, year = 2002 }

  %0 Journal Article %1 RN26 %A Langer, K. %A Ache, P. %A Geiger, D. %A Stinzing, A. %A Arend, M. %A Wind, C. %A Regan, S. %A Fromm, J. %A Hedrich, R. %D 2002 %J Plant J %P 997-1009 %T Poplar potassium transporters capable of controlling K+ homeostasis and K+-dependent xylogenesis %U http://www.ncbi.nlm.nih.gov/pubmed/12492841 %V 32
• Reintanz, B., Szyroki, A., Ivashikina, N., Ache, P., Godde, M., Becker, D., Palme, K., und Hedrich, R. (2002) „AtKC1, a silent Arabidopsis potassium channel alpha -subunit modulates root hair K+ influx“, Proc Natl Acad Sci U S A, 99, 4079–84, verfügbar unter: https://doi.org/10.1073/pnas.052677799.
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  @article{RN34, author = {Reintanz, B. and Szyroki, A. and Ivashikina, N. and Ache, P. and Godde, M. and Becker, D. and Palme, K. and Hedrich, R.}, journal = {Proc Natl Acad Sci U S A}, keywords = {myown}, pages = {4079-84}, title = {AtKC1, a silent Arabidopsis potassium channel alpha -subunit modulates root hair K+ influx}, type = {Journal Article}, volume = 99, year = 2002 }

  %0 Journal Article %1 RN34 %A Reintanz, B. %A Szyroki, A. %A Ivashikina, N. %A Ache, P. %A Godde, M. %A Becker, D. %A Palme, K. %A Hedrich, R. %D 2002 %J Proc Natl Acad Sci U S A %P 4079-84 %R 10.1073/pnas.052677799 %T AtKC1, a silent Arabidopsis potassium channel alpha -subunit modulates root hair K+ influx %U http://www.ncbi.nlm.nih.gov/pubmed/11904452 %V 99
• Deeken, R., Geiger, D., Fromm, J., Koroleva, O., Ache, P., Langenfeld-Heyser, R., Sauer, N., May, S.T., und Hedrich, R. (2002) „Loss of the AKT2/3 potassium channel affects sugar loading into the phloem of Arabidopsis“, Planta, 216, 334–44, verfügbar unter: https://doi.org/10.1007/s00425-002-0895-1.
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  @article{RN12, author = {Deeken, R. and Geiger, D. and Fromm, J. and Koroleva, O. and Ache, P. and Langenfeld-Heyser, R. and Sauer, N. and May, S. T. and Hedrich, R.}, journal = {Planta}, keywords = {myown}, pages = {334-44}, title = {Loss of the AKT2/3 potassium channel affects sugar loading into the phloem of Arabidopsis}, type = {Journal Article}, volume = 216, year = 2002 }

  %0 Journal Article %1 RN12 %A Deeken, R. %A Geiger, D. %A Fromm, J. %A Koroleva, O. %A Ache, P. %A Langenfeld-Heyser, R. %A Sauer, N. %A May, S. T. %A Hedrich, R. %D 2002 %J Planta %P 334-44 %R 10.1007/s00425-002-0895-1 %T Loss of the AKT2/3 potassium channel affects sugar loading into the phloem of Arabidopsis %U http://www.ncbi.nlm.nih.gov/pubmed/12447548 %V 216
• Schonknecht, G., Spoormaker, P., Steinmeyer, R., Bruggeman, L., Ache, P., Dutta, R., Reintanz, B., Godde, M., Hedrich, R., und Palme, K. (2002) „KCO1 is a component of the slow-vacuolar (SV) ion channel“, FEBS lett, 511(1-3), 28–32, verfügbar unter: http://www.ncbi.nlm.nih.gov/pubmed/11821043.
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  @article{RN36, author = {Schonknecht, G. and Spoormaker, P. and Steinmeyer, R. and Bruggeman, L. and Ache, P. and Dutta, R. and Reintanz, B. and Godde, M. and Hedrich, R. and Palme, K.}, journal = {FEBS lett}, keywords = {myown}, number = {1-3}, pages = {28-32}, title = {KCO1 is a component of the slow-vacuolar (SV) ion channel}, type = {Journal Article}, volume = 511, year = 2002 }

  %0 Journal Article %1 RN36 %A Schonknecht, G. %A Spoormaker, P. %A Steinmeyer, R. %A Bruggeman, L. %A Ache, P. %A Dutta, R. %A Reintanz, B. %A Godde, M. %A Hedrich, R. %A Palme, K. %D 2002 %J FEBS lett %N 1-3 %P 28-32 %T KCO1 is a component of the slow-vacuolar (SV) ion channel %U http://www.ncbi.nlm.nih.gov/pubmed/11821043 %V 511

• Ache, P., Becker, D., Deeken, R., Dreyer, I., Weber, H., Fromm, J., und Hedrich, R. (2001) „VFK1, a Vicia faba K+ channel involved in phloem unloading“, Plant J, 27, 571–80, verfügbar unter: http://www.ncbi.nlm.nih.gov/pubmed/11576440.
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  @article{RN2, author = {Ache, P. and Becker, D. and Deeken, R. and Dreyer, I. and Weber, H. and Fromm, J. and Hedrich, R.}, journal = {Plant J}, keywords = {myown}, pages = {571-80}, title = {VFK1, a Vicia faba K+ channel involved in phloem unloading}, type = {Journal Article}, volume = 27, year = 2001 }

  %0 Journal Article %1 RN2 %A Ache, P. %A Becker, D. %A Deeken, R. %A Dreyer, I. %A Weber, H. %A Fromm, J. %A Hedrich, R. %D 2001 %J Plant J %P 571-80 %T VFK1, a Vicia faba K+ channel involved in phloem unloading %U http://www.ncbi.nlm.nih.gov/pubmed/11576440 %V 27
• Ivashikina, N., Becker, D., Ache, P., Meyerhoff, O., Felle, H.H., und Hedrich, R. (2001) „K+ channel profile and electrical properties of Arabidopsis root hairs“, FEBS Lett, 508, 463–9, verfügbar unter: https://www.ncbi.nlm.nih.gov/pubmed/11728473.
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  @article{RN22, author = {Ivashikina, N. and Becker, D. and Ache, P. and Meyerhoff, O. and Felle, H. H. and Hedrich, R.}, journal = {FEBS Lett}, keywords = {myown}, pages = {463-9}, title = {K+ channel profile and electrical properties of Arabidopsis root hairs}, type = {Journal Article}, volume = 508, year = 2001 }

  %0 Journal Article %1 RN22 %A Ivashikina, N. %A Becker, D. %A Ache, P. %A Meyerhoff, O. %A Felle, H. H. %A Hedrich, R. %D 2001 %J FEBS Lett %P 463-9 %T K+ channel profile and electrical properties of Arabidopsis root hairs %U https://www.ncbi.nlm.nih.gov/pubmed/11728473 %V 508
• Szyroki, A., Ivashikina, N., Dietrich, P., Roelfsema, M.R., Ache, P., Reintanz, B., Deeken, R., Godde, M., Felle, H., Steinmeyer, R., Palme, K., und Hedrich, R. (2001) „KAT1 is not essential for stomatal opening“, Proc Natl Acad Sci U S A, 98, 2917–21, verfügbar unter: https://doi.org/10.1073/pnas.051616698.
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  @article{RN38, author = {Szyroki, A. and Ivashikina, N. and Dietrich, P. and Roelfsema, M. R. and Ache, P. and Reintanz, B. and Deeken, R. and Godde, M. and Felle, H. and Steinmeyer, R. and Palme, K. and Hedrich, R.}, journal = {Proc Natl Acad Sci U S A}, keywords = {myown}, pages = {2917-21}, title = {KAT1 is not essential for stomatal opening}, type = {Journal Article}, volume = 98, year = 2001 }

  %0 Journal Article %1 RN38 %A Szyroki, A. %A Ivashikina, N. %A Dietrich, P. %A Roelfsema, M. R. %A Ache, P. %A Reintanz, B. %A Deeken, R. %A Godde, M. %A Felle, H. %A Steinmeyer, R. %A Palme, K. %A Hedrich, R. %D 2001 %J Proc Natl Acad Sci U S A %P 2917-21 %R 10.1073/pnas.051616698 %T KAT1 is not essential for stomatal opening %U http://www.ncbi.nlm.nih.gov/pubmed/11226341 %V 98

• Deeken, R., Sanders, C., Ache, P., und Hedrich, R. (2000) „Developmental and light-dependent regulation of a phloem-localised K+ channel of Arabidopsis thaliana“, Plant J, 23, 285–90, verfügbar unter: http://www.ncbi.nlm.nih.gov/pubmed/10929122.
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  @article{RN14, author = {Deeken, R. and Sanders, C. and Ache, P. and Hedrich, R.}, journal = {Plant J}, keywords = {myown}, pages = {285-90}, title = {Developmental and light-dependent regulation of a phloem-localised K+ channel of Arabidopsis thaliana}, type = {Journal Article}, volume = 23, year = 2000 }

  %0 Journal Article %1 RN14 %A Deeken, R. %A Sanders, C. %A Ache, P. %A Hedrich, R. %D 2000 %J Plant J %P 285-90 %T Developmental and light-dependent regulation of a phloem-localised K+ channel of Arabidopsis thaliana %U http://www.ncbi.nlm.nih.gov/pubmed/10929122 %V 23
• Downey, P., Szabo, I., Ivashikina, N., Negro, A., Guzzo, F., Ache, P., Hedrich, R., Terzi, M., und Schiavo, F.L. (2000) „KDC1, a novel carrot root hair K+ channel. Cloning, characterization, and expression in mammalian cells“, J Biol Chem, 275, 39420–6, verfügbar unter: https://doi.org/10.1074/jbc.M002962200.
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  @article{RN15, author = {Downey, P. and Szabo, I. and Ivashikina, N. and Negro, A. and Guzzo, F. and Ache, P. and Hedrich, R. and Terzi, M. and Schiavo, F. L.}, journal = {J Biol Chem}, keywords = {myown}, pages = {39420-6}, title = {KDC1, a novel carrot root hair K+ channel. Cloning, characterization, and expression in mammalian cells}, type = {Journal Article}, volume = 275, year = 2000 }

  %0 Journal Article %1 RN15 %A Downey, P. %A Szabo, I. %A Ivashikina, N. %A Negro, A. %A Guzzo, F. %A Ache, P. %A Hedrich, R. %A Terzi, M. %A Schiavo, F. L. %D 2000 %J J Biol Chem %P 39420-6 %R 10.1074/jbc.M002962200 %T KDC1, a novel carrot root hair K+ channel. Cloning, characterization, and expression in mammalian cells %U http://www.ncbi.nlm.nih.gov/pubmed/10970888 %V 275
• Ache, P., Becker, D., Ivashikina, N., Dietrich, P., Roelfsema, M.R., und Hedrich, R. (2000) „GORK, a delayed outward rectifier expressed in guard cells of Arabidopsis thaliana, is a K+-selective, K+-sensing ion channel“, FEBS Lett, 486, 93–8, verfügbar unter: https://www.ncbi.nlm.nih.gov/pubmed/11113445.
  • [ BibTeX ]
  • [ EndNote ]
  • [ URL ]
  @article{RN3, author = {Ache, P. and Becker, D. and Ivashikina, N. and Dietrich, P. and Roelfsema, M. R. and Hedrich, R.}, journal = {FEBS Lett}, keywords = {myown}, pages = {93-8}, title = {GORK, a delayed outward rectifier expressed in guard cells of Arabidopsis thaliana, is a K+-selective, K+-sensing ion channel}, type = {Journal Article}, volume = 486, year = 2000 }

  %0 Journal Article %1 RN3 %A Ache, P. %A Becker, D. %A Ivashikina, N. %A Dietrich, P. %A Roelfsema, M. R. %A Hedrich, R. %D 2000 %J FEBS Lett %P 93-8 %T GORK, a delayed outward rectifier expressed in guard cells of Arabidopsis thaliana, is a K+-selective, K+-sensing ion channel %U https://www.ncbi.nlm.nih.gov/pubmed/11113445 %V 486

• Marten, I., Hoth, S., Deeken, R., Ache, P., Ketchum, K.A., Hoshi, T., und Hedrich, R. (1999) „AKT3, a phloem-localized K+ channel, is blocked by protons“, Proc Natl Acad Sci U S A, 96, 7581–6, verfügbar unter: http://www.ncbi.nlm.nih.gov/pubmed/10377458.
  • [ BibTeX ]
  • [ EndNote ]
  • [ URL ]
  @article{RN31, author = {Marten, I. and Hoth, S. and Deeken, R. and Ache, P. and Ketchum, K. A. and Hoshi, T. and Hedrich, R.}, journal = {Proc Natl Acad Sci U S A}, keywords = {myown}, pages = {7581-6}, title = {AKT3, a phloem-localized K+ channel, is blocked by protons}, type = {Journal Article}, volume = 96, year = 1999 }

  %0 Journal Article %1 RN31 %A Marten, I. %A Hoth, S. %A Deeken, R. %A Ache, P. %A Ketchum, K. A. %A Hoshi, T. %A Hedrich, R. %D 1999 %J Proc Natl Acad Sci U S A %P 7581-6 %T AKT3, a phloem-localized K+ channel, is blocked by protons %U http://www.ncbi.nlm.nih.gov/pubmed/10377458 %V 96